Yong D C, Smitka C, Prytula A, Kane J
Health Lab Sci. 1978 Oct;15(4):197-200.
A simple (2% oxgall) agar medium is described for the production of both germ tubes and chlamydospores by over 500 clinical isolates of Candida albicans. In comparison studies between the 2% oxgall agar and the more complex "cream of rice" infusion-oxgall-Tween 80 agar (RIOT), germ tubes were formed by 478 isolates in both media, by 9 isolates in only the oxgall medium and by 11 isolates in only the RIOT medium. Chlamydospores were formed by 481 isolates in both media, by 2 isolates in only the oxgall medium and by 9 isolates in only the RIOT medium. The data also show that both the germ tubes and chlamydospores were needed for the presumptive clinical identification of C. albicans.
本文描述了一种简单的(2%牛胆汁)琼脂培养基,用于500多株白色念珠菌临床分离株产生芽管和厚壁孢子。在2%牛胆汁琼脂与更复杂的“米糊”浸液-牛胆汁-吐温80琼脂(RIOT)的对比研究中,两种培养基中478株分离株形成了芽管,仅牛胆汁培养基中有9株形成芽管,仅RIOT培养基中有11株形成芽管。两种培养基中481株分离株形成了厚壁孢子,仅牛胆汁培养基中有2株形成厚壁孢子,仅RIOT培养基中有9株形成厚壁孢子。数据还表明,芽管和厚壁孢子对于白色念珠菌的临床初步鉴定都是必需的。