Yu Xiongtao, Bai Silan, Wang Lishi
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
Front Bioeng Biotechnol. 2023 Mar 17;11:1176046. doi: 10.3389/fbioe.2023.1176046. eCollection 2023.
In this work, gold nanoparticles@TiC MXenes nanocomposites with excellent properties were combined with toehold-mediated DNA strand displacement reaction to construct an electrochemical circulating tumor DNA biosensor. The gold nanoparticles were synthesized on the surface of TiC MXenes as a reducing and stabilizing agent. The good electrical conductivity of the gold nanoparticles@TiC MXenes composite and the nucleic acid amplification strategy of enzyme-free toehold-mediated DNA strand displacement reaction can be used to efficiently and specifically detect the non-small cell cancer biomarker circulating tumor DNA KRAS gene. The biosensor has a linear detection range of 10 fM -10 nM and a detection limit of 0.38 fM, and also efficiently distinguishes single base mismatched DNA sequences. The biosensor has been successfully used for the sensitive detection of KRAS gene G12D, which has excellent potential for clinical analysis and provides a new idea for the preparation of novel MXenes-based two-dimensional composites and their application in electrochemical DNA biosensors.
在这项工作中，将具有优异性能的金纳米粒子@TiC MXene纳米复合材料与基于核酸外切酶介导的DNA链置换反应相结合，构建了一种电化学循环肿瘤DNA生物传感器。金纳米粒子在TiC MXene表面合成，作为还原剂和稳定剂。金纳米粒子@TiC MXene复合材料良好的导电性以及无酶核酸外切酶介导的DNA链置换反应的核酸扩增策略，可用于高效、特异性地检测非小细胞癌生物标志物循环肿瘤DNA KRAS基因。该生物传感器的线性检测范围为10 fM - 10 nM，检测限为0.38 fM，还能有效区分单碱基错配的DNA序列。该生物传感器已成功用于KRAS基因G12D的灵敏检测，在临床分析方面具有优异潜力，并为新型MXene基二维复合材料的制备及其在电化学DNA生物传感器中的应用提供了新思路。
