电子烟液和醛类调味剂抑制肺上皮细胞中的CYP2A6活性。
E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells.
作者信息
Winters Brett R, Clapp Phillip W, Simmons Steven O, Kochar Tavleen K, Jaspers Ilona, Madden Michael C
机构信息
Curriculum in Toxicology and Environmental Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States.
Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States.
出版信息
ACS Omega. 2023 Mar 13;8(12):11261-11266. doi: 10.1021/acsomega.2c08258. eCollection 2023 Mar 28.
Certain e-liquids and aromatic aldehyde flavoring agents were previously identified as inhibitors of microsomal recombinant CYP2A6, the primary nicotine-metabolizing enzyme. However, due to their reactive nature, aldehydes may react with cellular components before reaching CYP2A6 in the endoplasmic reticulum. To determine whether e-liquid flavoring agents inhibited CYP2A6 in a cellular system, we investigated their effects on CYP2A6 using BEAS-2B cells transduced to overexpress CYP2A6. We demonstrated that two e-liquids and three aldehyde flavoring agents (cinnamaldehyde, benzaldehyde, and ethyl vanillin) exhibited dose-dependent inhibition of cellular CYP2A6.
某些电子烟液和芳香醛调味剂先前被确定为微粒体重组CYP2A6（主要的尼古丁代谢酶）的抑制剂。然而，由于醛类具有反应活性，它们可能在内质网中到达CYP2A6之前就与细胞成分发生反应。为了确定电子烟液调味剂在细胞系统中是否抑制CYP2A6，我们使用转导以过表达CYP2A6的BEAS-2B细胞研究了它们对CYP2A6的影响。我们证明了两种电子烟液和三种醛类调味剂（肉桂醛、苯甲醛和乙基香兰素）对细胞CYP2A6表现出剂量依赖性抑制作用。
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