Pradhan Rojalin, Kumar Shubham, Mathew Renjith
School of Biological Sciences, National Institute of Science Education and Research (NISER), Jatni, Khurda, Odisha 752050, India.
Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai 400094, India.
iScience. 2023 Mar 13;26(4):106380. doi: 10.1016/j.isci.2023.106380. eCollection 2023 Apr 21.
trachea is a classical model for analyzing epithelial, especially tubular epithelial biology. We identify lateral E-cadherin mediated junctions that encircle the cells just basal to the zonula adherens in the larval trachea. The lateral junction is associated with downstream adapters, including catenins, and has a distinct junctional actin cortex. The lateral cortex contributes to the development of a supracellular actomyosin mesh in the late larvae. Establishment of this cytoskeletal structure depends on lateral junction associated Rho1 and Cdc42 GTPases, and Arp and WASP pathways. The supracellular network takes the character of stress fibers along the AP axis in the early hours of pupation. It contributes to the shortening of the epithelial tube albeit in a manner redundant to ECM-mediated compression mechanism. In conclusion, we show the existence of functional lateral adherens junction and suggest a role for it in mediating dynamic cytoskeletal events during tissue scale morphogenesis.
气管是分析上皮细胞，尤其是管状上皮细胞生物学的经典模型。我们在幼虫气管中确定了侧向E-钙黏蛋白介导的连接，这些连接环绕着紧密连接下方的细胞。侧向连接与下游衔接蛋白相关，包括连环蛋白，并具有独特的连接肌动蛋白皮层。侧向皮层有助于晚期幼虫中超细胞肌动球蛋白网的发育。这种细胞骨架结构的建立依赖于与侧向连接相关的Rho1和Cdc42 GTP酶，以及Arp和WASP途径。在化蛹初期，超细胞网络呈现出沿前后轴的应力纤维特征。它有助于上皮管的缩短，尽管其方式与细胞外基质介导的压缩机制冗余。总之，我们证明了功能性侧向黏着连接的存在，并提出其在组织尺度形态发生过程中介导动态细胞骨架事件的作用。
