苹果酸合酶在两栖类脂肪体脂肪细胞中的细胞化学定位：脊椎动物中可能存在的乙醛酸循环

Cytochemical localization of malate synthase in amphibian fat body adipocytes: possible glyoxylate cycle in a vertebrate.

作者信息

Davis W L, Jones R G, Goodman D B

出版信息

J Histochem Cytochem. 1986 May;34(5):689-92. doi: 10.1177/34.5.3701032.

DOI:10.1177/34.5.3701032

Abstract

The adipocytes of amphibian abdominal fat bodies contain typical microperoxisomes, as indicated by their fine structure. Electron microscopic cytochemistry showed that these organelles contain the enzymes catalase, typical for peroxisomes, and malate synthase. The latter is an enzymatic component characteristic of the glyoxylate cycle, a biochemical pathway known to exist in plant glyoxysomes (peroxisomes). This metabolic pathway makes possible the net conversion of lipid to carbohydrate. Toad adipocytes may represent yet another example of vertebrate peroxisomes which contain one of the marker enzymes (malate synthase) characteristic of the glyoxylate shunt.

摘要

两栖动物腹部脂肪体的脂肪细胞含有典型的微过氧化物酶体，这从其精细结构可以看出。电子显微镜细胞化学显示，这些细胞器含有过氧化氢酶（过氧化物酶体的典型酶）和苹果酸合酶。后者是乙醛酸循环的一种酶成分，乙醛酸循环是一种已知存在于植物乙醛酸循环体（过氧化物酶体）中的生化途径。这种代谢途径使得脂质净转化为碳水化合物成为可能。蟾蜍脂肪细胞可能代表了脊椎动物过氧化物酶体的又一个例子，其含有乙醛酸分流的一种标志性酶（苹果酸合酶）。