Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, People's Republic of China.
Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
J Agric Food Chem. 2023 Apr 12;71(14):5851-5860. doi: 10.1021/acs.jafc.3c00151. Epub 2023 Apr 3.
In this study, a novel pseudo-targeted peptidomics strategy, integrating the transition list generated by an in-house software (Pep-MRMer) and the retention time transfer by high-abundance ion-based retention time calibration (HAI-RT-cal), was developed to screen marker peptides of gelatins from five closely related animal species, including porcine, bovine, horse, mule, and donkey. Five marker peptides were screened from the molecular phenotypic differences of type I collagen. Furthermore, a simple and robust 10 min multiple reaction monitoring (MRM) method was established and performed well in distinguishing different gelatins, particularly in discerning horse-hide gelatin (HHG) and mule-hide gelatin (MHG) from donkey-hide gelatin (DHG). The market investigation revealed the serious adulteration of DHG. Meantime, the pseudo-targeted peptidomics could be used to screen marker peptides of other gelatin foods.
本研究建立了一种新型的拟靶向肽组学策略，整合了内部软件（Pep-MRMer）生成的转换列表和基于高丰度离子的保留时间转移的保留时间校准（HAI-RT-cal），用于筛选来自 5 种密切相关的动物物种（包括猪、牛、马、骡和驴）的明胶的标记肽。从 I 型胶原蛋白的分子表型差异中筛选出 5 个标记肽。此外，建立了一种简单、稳健的 10 分钟多重反应监测（MRM）方法，可用于区分不同的明胶，特别是用于区分马皮明胶（HHG）和骡皮明胶（MHG）与驴皮明胶（DHG）。市场调查显示 DHG 存在严重掺假的情况。同时，拟靶向肽组学可用于筛选其他明胶食品的标记肽。
