大鼠单克隆抗体。VI. 产生对2,4-二硝基苯基（DNP）半抗原具有特异性的分泌IgA的杂交瘤。
Rat monoclonal antibodies. VI. Production of IgA secreting hybridomas with specificity for the 2,4-dinitrophenyl (DNP) hapten.
作者信息
Rits M, Cormont F, Bazin H, Meykens R, Vaerman J P
出版信息
J Immunol Methods. 1986 May 1;89(1):81-7. doi: 10.1016/0022-1759(86)90034-7.
A simple method to obtain rat hybridomas producing specific IgA antibodies is reported. By fusing the IR983F rat myeloma cell line with mesenteric lymph node cells from LOU/C rats immunized via the Peyer's patches with DNP-Salmonella typhimurium, twenty hybrids secreting monoclonal IgA antibodies specific for DNP were produced and maintained as highly secreting transplantable ascitic tumors. The monoclonal IgA antibodies were easily purified by affinity chromatography on a DNP-immunosorbent and were found to comprise both monomers and polymers.
报道了一种获得产生特异性IgA抗体的大鼠杂交瘤的简单方法。通过将IR983F大鼠骨髓瘤细胞系与经派伊尔结用二硝基苯基 - 鼠伤寒沙门氏菌免疫的LOU/C大鼠的肠系膜淋巴结细胞融合，产生了20个分泌针对二硝基苯基的单克隆IgA抗体的杂交瘤，并作为高分泌性可移植腹水瘤进行维持。单克隆IgA抗体通过在二硝基苯基免疫吸附剂上的亲和层析很容易纯化，并且发现其包含单体和聚合物。
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