Ju Shinyeong, Lee Cheolju
Chemical & Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Seoul, Republic of Korea.
Methods Mol Biol. 2023;2620:229-241. doi: 10.1007/978-1-0716-2942-0_25.
Mass spectrometric analysis of N-terminal peptides reveals altered amino acid sequences at the protein's N-terminus and the presence of posttranslational modifications (PTM). Recent advancement in enriching N-terminal peptides facilitates the discovery of rare N-terminal PTMs in samples with restricted availability. In this chapter, we describe a simple, single-stage oriented N-terminal peptide enrichment method that helps the overall sensitivity of N-terminal peptides. In addition, we describe how to increase the depth of identification, to use software to identify and quantify N-terminally arginylated peptides.
对N端肽段的质谱分析揭示了蛋白质N端氨基酸序列的改变以及翻译后修饰（PTM）的存在。富集N端肽段的最新进展有助于在样本量有限的情况下发现罕见的N端PTM。在本章中，我们描述了一种简单的单阶段定向N端肽段富集方法，该方法有助于提高N端肽段的整体灵敏度。此外，我们还描述了如何增加鉴定深度，以及如何使用软件来鉴定和定量N端精氨酸化肽段。
