Yuan Cheng, Xu Yi-Tong, Huang Yu-Ting, Zhou Hong, Jiang Xing-Wu, Ju Peng, Zhu Yuan-Cheng, Zhang Ling, Lin Peng, Chen Guangxu, Zhao Wei-Wei
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China.
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China.
ACS Sens. 2023 Apr 28;8(4):1835-1840. doi: 10.1021/acssensors.3c00289. Epub 2023 Apr 3.
Organic photoelectrochemical transistor (OPECT) biosensing represents a new platform interfacing optoelectronics and biological systems with essential amplification, which, nevertheless, are concentrated on depletion-type operation to date. Here, a polymer dot (Pdot)-gated accumulation-type OPECT biosensor is devised and applied for sensitive urea detection. In such a device, the as-designed Pdot/poly[bis(4-phenyl)(2,4,6-trimethylphenyl)amine] (PTAA) is validated as a superior gating module against the diethylenetriamine (DETA) de-doped poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel, and the urea-dependent status of Pdots has been shown to be sensitively correlated with the device's response. High-performance urea detection is thus realized with a wide linear range of 1 μM-50 mM and a low detection limit of 195 nM. Given the diversity of the Pdot family and its immense interactions with other species, this work represents a generic platform for developing advanced accumulation-type OPECT and beyond.
有机光电化学晶体管（OPECT）生物传感代表了一个连接光电子学和生物系统的新平台，具有重要的信号放大功能，然而，迄今为止其主要集中在耗尽型操作上。在此，设计了一种聚合物点（Pdot）栅控积累型OPECT生物传感器并将其应用于灵敏的尿素检测。在这种器件中，所设计的Pdot/聚[双（4-苯基）（2,4,6-三甲基苯基）胺]（PTAA）被证实是一种优于二亚乙基三胺（DETA）去掺杂的聚（3,4-亚乙基二氧噻吩）：聚（苯乙烯磺酸盐）（PEDOT:PSS）通道的栅控模块，并且已表明Pdot的尿素依赖状态与器件响应灵敏相关。因此实现了高性能的尿素检测，线性范围宽达1 μM - 50 mM，检测限低至195 nM。鉴于Pdot家族的多样性及其与其他物种的巨大相互作用，这项工作代表了一个用于开发先进的积累型OPECT及其他应用的通用平台。
