State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
Department of Horticulture and Landscape Gardening, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila 60170 Sri Lanka.
J Agric Food Chem. 2024 Aug 14;72(32):17746-17761. doi: 10.1021/acs.jafc.4c04191. Epub 2024 Jul 30.
In order to solve the food safety problem better, it is very important to develop a rapid and sensitive technology for detecting food contamination residues. Organic photoelectrochemical transistor (OPECT) biosensor rely on the photovoltage generated by a semiconductor upon excitation by light to regulate the conductivity of the polymer channels and realize biosensor analysis under zero gate bias. This technology integrates the excellent characteristics of photoelectrochemical (PEC) bioanalysis and the high sensitivity and inherent amplification ability of organic electrochemical transistor (OECT). Based on this, OPECT biosensor detection has been proven to be superior to traditional biosensor detection methods. In this review, we summarize the research status of OPECT biosensor in disease markers and food residue analysis, the basic principle, classification, and biosensing mechanism of OPECT biosensor analysis are briefly introduced, and the recent applications of biosensor analysis are discussed according to the signal strategy. We mainly introduced the OPECT biosensor analysis methods applied in different fields, including the detection of disease markers and food hazard residues such as prostate-specific antigen, heart-type fatty acid binding protein, T-2 toxin detection in milk samples, fat mass and objectivity related protein, ciprofloxacin in milk. The OPECT biosensor provides considerable development potential for the construction of safety analysis and detection platforms in many fields, such as agriculture and food, and hopes to provide some reference for the future development of biosensing analysis methods with higher selectivity, faster analysis speed and higher sensitivity.
为了更好地解决食品安全问题，开发一种用于检测食物污染残留的快速灵敏技术非常重要。有机光电化学晶体管（OPECT）生物传感器依赖于半导体在光激发下产生的光电压来调节聚合物通道的电导率，并在零栅极偏压下实现生物传感器分析。该技术集成了光电化学（PEC）生物分析的优异特性以及有机电化学晶体管（OECT）的高灵敏度和固有放大能力。基于此，OPECT 生物传感器检测已被证明优于传统生物传感器检测方法。在这篇综述中，我们总结了 OPECT 生物传感器在疾病标志物和食物残留分析中的研究现状，简要介绍了 OPECT 生物传感器的基本原理、分类和生物传感机制，并根据信号策略讨论了生物传感器分析的最新应用。我们主要介绍了应用于不同领域的 OPECT 生物传感器分析方法，包括对疾病标志物和食品安全分析检测领域的前列腺特异性抗原、人心型脂肪酸结合蛋白、牛奶样品中 T-2 毒素、脂肪质量和客观性相关蛋白、牛奶中的环丙沙星等的检测。OPECT 生物传感器为农业和食品等许多领域的安全分析和检测平台的构建提供了相当大的发展潜力，希望为未来开发具有更高选择性、更快分析速度和更高灵敏度的生物传感分析方法提供一些参考。
