Key Laboratory of Optical-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Anal Chem. 2023 Aug 8;95(31):11800-11806. doi: 10.1021/acs.analchem.3c02258. Epub 2023 Jul 28.
Organic photoelectrochemical transistor (OPECT) biosensor with a removed background is desired but remains challenging. So far, scientists still lack a solution to this issue. The light-matter interplay is expected to achieve an advanced OPECT with unknown possibilities. Here, we address this challenge by tailoring a unique heterogeneous light antenna as the functional gating module and its cascade interaction with a proper channel, which is exemplified by bioinduced [Ru(bpy)dppz]-intercalated DNA nanotubes (NTs)/NiO heterojunction and its modulation against a diethylenetriamine-treated poly(ethylene dioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) channel. Light stimulation of the antenna can generate the obvious cathodic photocurrent and, hence, modulate the channel, accomplishing OPECT with a minimal background and the hitherto highest current gain of 19 000. Linking with nucleic acid hybridization using microRNA-155 as the representative target, the device achieves sensitive biosensing down to 5.0 fM.
有机光电化学晶体管（OPECT）生物传感器需要去除背景，但这仍然具有挑战性。到目前为止，科学家们仍然没有解决这个问题的办法。光与物质的相互作用有望实现具有未知可能性的先进 OPECT。在这里，我们通过定制独特的异质光天线作为功能门控模块及其与适当的通道的级联相互作用来解决这一挑战，这在生物诱导的[Ru（bpy）dppz]嵌入 DNA 纳米管（NTs）/NiO 异质结及其对二乙三胺处理的聚（乙二氧基噻吩）：聚（苯乙烯磺酸盐）（PEDOT：PSS）通道的调制中得到了例证。天线的光刺激可以产生明显的阴极光电流，从而调制通道，实现具有最小背景和迄今为止最高电流增益 19000 的 OPECT。通过使用 microRNA-155 作为代表性靶标进行核酸杂交，该器件实现了低至 5.0 fM 的灵敏生物传感。
