Lin Zhiwen, Tan Jinan, Xiong Zhihan, Fu Zisen, Chen Jing, Xie Tonghui, Zheng Jia, Zhang Yongkui, Li Panyu
Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, PR China.
Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry, Yibin, Sichuan 644007, PR China.
Bioresour Technol. 2023 Jun;378:129011. doi: 10.1016/j.biortech.2023.129011. Epub 2023 Apr 1.
The autochthonous microbial community from excess sludge was regulated for enhanced conversion of CO to acetate without exogenic H. It was interesting that the acetate-fed system exhibited a surprising performance to regulate the microbial community for a high acetate yield and selectivity. As a result, some hydrogen-producing bacteria (e.g., Proteiniborus) and acetogenic bacteria with the ability of CO reduction were enriched by acetate feeding, 2-bromoethanesulfonate (BES) addition and CO stress. When the selected microbial community was applied to convert CO, the accumulation of acetate was positively correlated to the concentration of yeast extract. Finally, the acetate yield reached up to 67.24 mM with a high product selectivity of 84 % in the presence of yeast extract (2 g/L) and sufficient CO in semi-continuous culture for 10 days. This work should help get new insights into the regulation of microbial community for the efficient acetate production from CO.
对剩余污泥中的本地微生物群落进行调控，以在无外源氢气的情况下增强一氧化碳向乙酸盐的转化。有趣的是，以乙酸盐为进料的系统在调控微生物群落以实现高乙酸盐产量和选择性方面表现出惊人的性能。结果，通过乙酸盐进料、添加2-溴乙烷磺酸盐（BES）和一氧化碳胁迫，一些产氢细菌（如蛋白杆菌属）和具有一氧化碳还原能力的产乙酸细菌得以富集。当将选定的微生物群落用于转化一氧化碳时，乙酸盐的积累与酵母提取物的浓度呈正相关。最后，在酵母提取物（2 g/L）存在且一氧化碳充足的半连续培养10天的条件下，乙酸盐产量达到67.24 mM，产品选择性高达84%。这项工作应有助于深入了解微生物群落调控以实现由一氧化碳高效生产乙酸盐的过程。
