Said H M, Blair J A, Lucas M L, Hilburn M E
J Lab Clin Med. 1986 May;107(5):420-4.
The presence of the intestinal surface acid microclimate (ISAM) has long been suggested. Controversy, however, still exists as to the existence, general characteristics, and requirements of this microclimate. We examined the existence and distribution of the ISAM along the rat small intestine and colon and determined its general characteristics. The results clearly demonstrate the existence of the ISAM in vitro and in vivo. Glucose and sodium in the incubation medium, as well as normal intracellular metabolism, are essential requirements for the formation and maintenance of the ISAM. We conclude that the ISAM is a normal physiologic phenomenon of the luminal surface of the intestine.
长期以来，人们一直认为存在肠道表面酸性微气候（ISAM）。然而，关于这种微气候的存在、一般特征和条件仍存在争议。我们研究了ISAM在大鼠小肠和结肠中的存在及分布，并确定了其一般特征。结果清楚地证明了ISAM在体外和体内均存在。孵育培养基中的葡萄糖和钠以及正常的细胞内代谢是ISAM形成和维持的必要条件。我们得出结论，ISAM是肠道腔表面的一种正常生理现象。
