Miles Shawna, Lee Cameron, Breeden Linda
Basic Science, Fred Hutchinson Cancer Center, Seattle, Washington, United States.
Tune Therapeutics, 1930 Boren Ave., Seattle, Washington, United States.
MicroPubl Biol. 2023 Mar 17;2023. doi: 10.17912/micropub.biology.000742. eCollection 2023.
In rich medium, W303 begins to accumulate in G1 an hour before it exhausts the available glucose. It undergoes one more asymmetrical cell division, then stops dividing in G1. In contrast, BY4741, stops dividing four hours before glucose exhaustion, at one-fourth the cell density achieved by W303. There is no asymmetrical cell division and only 50% of the cells arrest in G1. We conclude that BY4741 growth is not limited by glucose and they do not go through the stereotypical events carried out by other strains as they enter quiescence from rich medium. In W303, the timing of glucose limitation and the transition to quiescence is correlated with the rate of biomass accumulation and cell doubling time.
在丰富培养基中，W303在耗尽可用葡萄糖前一小时开始在G1期积累。它再经历一次不对称细胞分裂，然后在G1期停止分裂。相比之下，BY4741在葡萄糖耗尽前四小时停止分裂，此时细胞密度仅为W303达到的四分之一。没有不对称细胞分裂，只有50%的细胞停滞在G1期。我们得出结论，BY4741的生长不受葡萄糖限制，并且当它们从丰富培养基进入静止期时，不会经历其他菌株所经历的典型事件。在W303中，葡萄糖限制的时间和向静止期的转变与生物量积累速率和细胞倍增时间相关。
