Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Nanjing, Nanjing, 210014, China.
Nanjing Forestry University, Nanjing, 210037, China.
BMC Biol. 2023 Apr 3;21(1):68. doi: 10.1186/s12915-023-01564-8.
The accumulation of fatty acids in plants covers a wide range of functions in plant physiology and thereby affects adaptations and characteristics of species. As the famous woody oilseed crop, Acer truncatum accumulates unsaturated fatty acids and could serve as the model to understand the regulation and trait formation in oil-accumulation crops. Here, we performed Ribosome footprint profiling combing with a multi-omics strategy towards vital time points during seed development, and finally constructed systematic profiling from transcription to proteomes. Additionally, we characterized the small open reading frames (ORFs) and revealed that the translational efficiencies of focused genes were highly influenced by their sequence features.
The comprehensive multi-omics analysis of lipid metabolism was conducted in A. truncatum. We applied the Ribo-seq and RNA-seq techniques, and the analyses of transcriptional and translational profiles of seeds collected at 85 and 115 DAF were compared. Key members of biosynthesis-related structural genes (LACS, FAD2, FAD3, and KCS) were characterized fully. More meaningfully, the regulators (MYB, ABI, bZIP, and Dof) were identified and revealed to affect lipid biosynthesis via post-translational regulations. The translational features results showed that translation efficiency tended to be lower for the genes with a translated uORF than for the genes with a non-translated uORF. They provide new insights into the global mechanisms underlying the developmental regulation of lipid metabolism.
We performed Ribosome footprint profiling combing with a multi-omics strategy in A. truncatum seed development, which provides an example of the use of Ribosome footprint profiling in deciphering the complex regulation network and will be useful for elucidating the metabolism of A. truncatum seed oil and the regulatory mechanisms.
植物中脂肪酸的积累涵盖了植物生理学的广泛功能，从而影响了物种的适应和特征。作为著名的木本油料作物，翅果油树积累了不饱和脂肪酸，可以作为理解油料作物中调控和性状形成的模式。在这里，我们通过核糖体足迹分析结合多组学策略，对种子发育过程中的关键时间点进行了研究，最终构建了从转录组到蛋白质组的系统分析。此外，我们还对小开放阅读框（ORFs）进行了特征分析，揭示了聚焦基因的翻译效率受到其序列特征的高度影响。
我们对翅果油树的脂代谢进行了全面的多组学分析。我们应用了 Ribo-seq 和 RNA-seq 技术，并比较了在 85 和 115 DAF 时收集的种子的转录组和翻译组谱分析。充分表征了生物合成相关结构基因（LACS、FAD2、FAD3 和 KCS）的关键成员。更有意义的是，我们鉴定并揭示了调控因子（MYB、ABI、bZIP 和 Dof）通过翻译后调控影响脂质生物合成。翻译特征结果表明，具有翻译 uORF 的基因的翻译效率往往低于具有非翻译 uORF 的基因。这些结果为脂代谢发育调控的全局机制提供了新的见解。
我们在翅果油树种子发育过程中进行了核糖体足迹分析结合多组学策略，为核糖体足迹分析在破译复杂调控网络中的应用提供了一个范例，并将有助于阐明翅果油树种子油的代谢和调控机制。
