CRISPR/Cas12a增强的单分子计数用于在单细胞水平灵敏检测瓣内切核酸酶1的活性。
CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level.
作者信息
Zhao Ning-Ning, Tian Xiaorui, Ma Fei, Zhang Chun-Yang
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
出版信息
Chem Commun (Camb). 2023 Apr 20;59(33):4939-4942. doi: 10.1039/d3cc00642e.
We develop a new fluorescent biosensor for flap endonuclease 1 (FEN1) assay based on CRISPR/Cas12-enhanced single-molecule counting. This biosensor is simple, selective, and sensitive with a detection limit of 2.325 × 10 U and it is applicable for inhibitor screening, kinetic parameter analysis, and quantifying cellular FEN1 with single-cell sensitivity.
我们基于CRISPR/Cas12增强的单分子计数技术开发了一种用于翼状内切核酸酶1（FEN1）检测的新型荧光生物传感器。这种生物传感器简单、具有选择性且灵敏，检测限为2.325×10 U，适用于抑制剂筛选、动力学参数分析以及以单细胞灵敏度对细胞内FEN1进行定量。
Zotero 插件