连接酶检测反应扩增激活 CRISPR-Cas12a 用于乳腺癌组织中 FEN1 的单分子计数。
Ligase detection reaction amplification-activated CRISPR-Cas12a for single-molecule counting of FEN1 in breast cancer tissues.
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China.
出版信息
Chem Commun (Camb). 2024 Mar 12;60(22):3075-3078. doi: 10.1039/d4cc00408f.
We construct a simple fluorescent biosensor for single-molecule counting of flap endonuclease 1 (FEN1) based on ligase detection reaction (LDR) amplification-activated CRISPR-Cas12a. This biosensor exhibits excellent selectivity and high sensitivity with a detection limit (LOD) of 1.31 × 10 U. Moreover, it can be employed to screen the FEN1 inhibitors and quantitatively measure the FEN1 activity in human cells and breast cancer tissues, holding great promise in clinical diagnosis and drug discovery.
我们构建了一种基于连接酶检测反应（LDR）放大激活 CRISPR-Cas12a 的简单荧光生物传感器，用于单分子计数解旋酶 1（FEN1）。该生物传感器具有优异的选择性和高灵敏度，检测限（LOD）为 1.31×10 U。此外，它可用于筛选 FEN1 抑制剂并定量测量人细胞和乳腺癌组织中的 FEN1 活性，在临床诊断和药物发现方面具有广阔的应用前景。
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