Kholy A E, Facklam R, Sabri G, Rotta J
J Clin Microbiol. 1978 Dec;8(6):725-8. doi: 10.1128/jcm.8.6.725-728.1978.
The use of a microtechnique (modified nitrous acid extraction) to test samples from 150 school children and from patients with acute follicular tonsillitis has indicated that group A streptococci in the throat can be identified from tonsillar scrapings in 30 min. The results are comparable to the grouping results obtained by standard throat culture techniques and the Lancefield procedure for grouping. No cross-reaction with other bacteria or cellular material occurs. Study has also shown that the nitrous acid extraction yields three- to fourfold more polysaccharides than the Lancefield hot-HCl of Fuller formamide techniques. The use of the microtechnique leads to another 20-fold concentration of the antigen. Immune salting-out effect could be obtained with 1.00 M sodium acetate. Such molarity is too low to cause nonspecific slating out. It leads to a strong ampliciation of the precipitin reaction.
使用一种微量技术（改良亚硝酸提取法）对150名学童和急性滤泡性扁桃体炎患者的样本进行检测，结果表明，30分钟内即可从扁桃体刮片中鉴定出咽喉中的A组链球菌。该结果与通过标准咽喉培养技术和兰斯菲尔德分组法获得的分组结果相当。未出现与其他细菌或细胞物质的交叉反应。研究还表明，与富勒甲酰胺技术的兰斯菲尔德热盐酸法相比，亚硝酸提取法产生的多糖多三到四倍。使用微量技术可使抗原再浓缩20倍。用1.00M醋酸钠可获得免疫盐析效应。这种摩尔浓度过低，不会导致非特异性盐析。它会使沉淀素反应强烈放大。
