George J R, Ashworth H, Facklam R R, Harrell W K, Palmer D F
J Clin Microbiol. 1981 Oct;14(4):433-6. doi: 10.1128/jcm.14.4.433-436.1981.
Antisera to streptococcal groups A through G containing 4% polyethylene glycol 6000 were prepared and evaluated. Seventy strains of homologous and heterologous beta- and non-beta-hemolytic streptococci were included in the evaluation. Homologous reactions were determined against extracts prepared by four extraction methods: hot hydrochloric acid (Lancefield) extraction, autoclave extraction, hot formamide extraction, and nitrous acid extraction. Enhancement of the precipitin reaction in the presence of polyethylene glycol 6000 permitted a fourfold dilution of all antisera for use in the capillary precipitin test. At this dilution, the potency of the antisera exceeded requirements established for these reagents.
制备并评估了含4%聚乙二醇6000的抗A至G组链球菌抗血清。评估中纳入了70株同源和异源的β溶血及非β溶血链球菌。针对通过四种提取方法制备的提取物测定同源反应：热盐酸（兰斯菲尔德）提取、高压灭菌提取、热甲酰胺提取和亚硝酸提取。聚乙二醇6000存在下沉淀素反应的增强使得所有抗血清在毛细管沉淀试验中可稀释四倍使用。在此稀释度下，抗血清的效价超过了为这些试剂设定的要求。
