Guinet R, André J, Barbé G, Boude M, Carret G, Freydiére A M, Gille Y, Marcel J P, Mazoyer M A
Med Microbiol Immunol. 1982;171(1):23-32. doi: 10.1007/BF02122704.
Three serological methods for streptococci grouping: latex agglutination after pronase extraction (LAP), latex agglutination after nitrous acid extraction (LAN) and counterimmunoelectrophoresis after nitrous acid extraction (CIEN) were evaluated in six laboratories with 310 strains of streptococci. To reduce the possible influence of a particular laboratory, each serological procedure was performed in two different laboratories with identical protocols and strains. Between the three procedures, a total agreement of 81.3% with six identical results was obtained. Since only one discordant result was obtained in 12.2% (CIEN 4.8%-LAN 5.8%-LAP 1.6%) and two discordant results with two different serological methods in 2.6%, it could be concluded that agreement between the three procedures was 96%. For B (99 strains tested) and D(100 strains tested) groups, total agreements of respectively 95% and 92% were achieved. In conclusion, LAP, LAN, and CIEN gave equivalent results for streptococci grouping, but LAN was the most simple needing no heating, centrifugation, or electrophoresis equipment and lacking cross-reactivity of group C antibody.
链霉蛋白酶提取后的乳胶凝集试验（LAP）、亚硝酸提取后的乳胶凝集试验（LAN）和亚硝酸提取后的对流免疫电泳（CIEN），在六个实验室中用310株链球菌进行了评估。为减少特定实验室可能产生的影响，每种血清学方法都在两个不同实验室按照相同方案和菌株进行操作。在这三种方法之间，获得了81.3%的完全一致结果，即有六个相同结果。由于在12.2%的情况中仅获得一个不一致结果（CIEN为4.8% - LAN为5.8% - LAP为1.6%），且在2.6%的情况中有两个不同血清学方法的不一致结果，所以可以得出这三种方法之间的一致性为96%。对于B组（检测了99株）和D组（检测了100株），分别达到了95%和92%的完全一致结果。总之，LAP、LAN和CIEN在链球菌分组方面给出了等效结果，但LAN最为简单，无需加热、离心或电泳设备，且缺乏C组抗体的交叉反应性。
