Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.
Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.
J Microbiol Methods. 2023 May;208:106709. doi: 10.1016/j.mimet.2023.106709. Epub 2023 Apr 2.
The poultry industry in developing countries is still combating mortality and economic loss due to Salmonella contamination. Salmonella Gallinarum is a common pathogen of poultry birds, being the etiologic agent of fowl typhoid, which specifically infects adult birds via the oral-fecal route. Timely detection of S. Gallinarum in poultry flocks can allow early treatment intervention leading to a decrease in economic losses. Detection of S. Gallinarum is challenging, while its PCR-based detection is a promising strategy, however, due to its high genomic similarity with other commonly existing Salmonella spp., identification of S. Gallinarum from poultry samples with high specificity is still a challenge. The current study was conducted to isolate S. Gallinarum from different districts of Pakistan, assess their antibiotic susceptibility profile, and develop a method for its early detection. A total of 20 strains were isolated using buffer peptone water, selenite cysteine broth, and Xylose Lysine Tergitol-4 (XLT-4) agar supplemented with tergitol and characterized by biochemical procedures. The antibiotic sensitivity profile highlighted the highest resistance of isolates towards novobiocin and nalidixic acid, commonly used antibiotics in Pakistan Poultry production. The primers designed to amplify a unique genomic region of S. Gallinarum, showed successful detection of twenty S. Gallinarum strains, while no amplification with genomic DNA from other common Salmonella spp. The reported method can be utilized to detect S. Gallinarum from tissue samples of infected birds in a short time leading to early diagnosis and timely treatment intervention.
发展中国家的家禽业仍在努力应对因沙门氏菌污染而导致的死亡率和经济损失。鸡白痢沙门氏菌是家禽鸟类的常见病原体，是禽伤寒的病原体，通过口腔-粪便途径专门感染成年鸟类。及时检测家禽群中的 S. Gallinarum 可以进行早期治疗干预，从而减少经济损失。检测 S. Gallinarum 具有挑战性，而基于 PCR 的检测是一种有前途的策略，但由于其与其他常见的沙门氏菌属高度相似，因此从家禽样本中高度特异性地鉴定 S. Gallinarum 仍然是一个挑战。本研究旨在从巴基斯坦不同地区分离 S. Gallinarum，评估其抗生素敏感性谱，并开发早期检测方法。使用缓冲蛋白胨水、亚硒酸盐胱氨酸肉汤和添加特里戈尔的木糖赖氨酸 Tergitol-4（XLT-4）琼脂从不同地区分离了 20 株 S. Gallinarum，并通过生化程序进行了鉴定。抗生素敏感性谱突出显示了分离株对诺氟沙星和萘啶酸的最高耐药性，这两种抗生素在巴基斯坦家禽生产中常用。设计用于扩增 S. Gallinarum 独特基因组区域的引物成功地检测了 20 株 S. Gallinarum 株，而其他常见沙门氏菌属的基因组 DNA 则没有扩增。所报道的方法可用于在短时间内从感染鸟类的组织样本中检测 S. Gallinarum，从而实现早期诊断和及时治疗干预。
