Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, C/Prof., García González S/N, 41012, Seville, Spain.
Mikrochim Acta. 2023 Apr 4;190(5):170. doi: 10.1007/s00604-023-05752-9.
A new microfluidic device to enhance the enrichment factor in miniaturized systems is proposed. The microfluidic system was design for liquid phase microextractions, and it was applied to the simultaneous extraction of acidic compounds of a wide range of polarity (0.5 < log P < 3). The device operated under stagnant acceptor phase conditions and all the operational parameters involved were optimized. Tributyl phosphate was found to be a new highly efficient supported liquid membrane to simultaneously extract analytes of very different polarities. The optimal donor and acceptor phase were pH 2 and pH 13, respectively. The donor flow rate and the extraction time were investigated simultaneously, offering great versatility with high enrichment factors (EFs). Limits of quantitation were within 0.02 and 0.09 µg mL for all compounds at 10 µL min as donor flow rate and 20-min extractions, offering EFs between 11 and 18 with only 200-µL sample volume consumption. The method was successfully applied to human urine samples, observing recoveries between 47 and 90% for all compounds. This new proposed microfluidic system increases the wide range of applications, especially when the analytes are present in lower concentrations in the sample.
提出了一种新的微流控装置，以提高微型化系统中的富集因子。该微流控系统专为液相微萃取而设计，并应用于对极性范围很宽的酸性化合物（0.5 < log P < 3）的同时萃取。该装置在停滞接受相条件下运行，优化了所有涉及的操作参数。三丁基磷酸酯被发现是一种新的高效支撑液膜，可同时萃取极性差异很大的分析物。最佳的供体和接受相分别为 pH 2 和 pH 13。同时考察了供体流速和萃取时间，提供了高富集因子（EF）的多功能性。在 10 µL min 的供体流速和 20 分钟的萃取时间下，所有化合物的定量限均在 0.02 和 0.09 µg mL 之间，EF 在 11 到 18 之间，仅消耗 200 µL 样品体积。该方法成功应用于人体尿液样品，所有化合物的回收率在 47%至 90%之间。这种新提出的微流控系统增加了广泛的应用，特别是当样品中分析物的浓度较低时。
