Laboratory of Cell-Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima, 30000, Thailand.
Mol Biotechnol. 2024 Jan;66(1):44-55. doi: 10.1007/s12033-023-00732-z. Epub 2023 Apr 4.
In the epidermal and dermal layers of the skin, diverse cell types are reconstituted during the wound healing process. Delays or failures in wound healing are a major issue in skin therapy because they prevent the normal structure and function of wounded tissue from being restored, resulting in ulceration or other skin abnormalities. Human immortalized keratinocytes (HaCAT) cells are a spontaneously immortalized human keratinocyte cell line capable of secreting many bioactive chemicals (a secretome) that stimulate skin cell proliferation, rejuvenation, and regeneration. In this study, the HaCaT secretome was encapsulated with polyesters such as poly (lactic-co-glycolic acid) (PLGA) and cassava starch in an effort to maximize its potential. According to the estimated mechanism of the HaCaT secretome, all treatments were conducted on immortalized dermal fibroblast cell lines, a model of wound healing. Encapsulation of HaCaT secretome and cassava starch enhanced the effectiveness of cell proliferation, migration, and anti-aging. On the other hand, the levels of reactive oxygen species (ROS) were lowered, activating antioxidants in immortalized dermal fibroblast cells. The HaCaT secretome induced in a dose-dependent manner the expression of antioxidant-associated genes, including SOD, CAT, and GPX. Six cytokines, including CCL2 and MCP-1, influenced immunoregulatory and inflammatory processes in cultured HaCAT cells. HaCaT secretome encapsulated in cassava starch can reduce ROS buildup by boosting antioxidant to stimulate wound healing. Hence, the HaCaT secretome may have a new chance in the cosmetics business to develop components for wound prevention and healing.
在皮肤的表皮和真皮层中，多种细胞类型在伤口愈合过程中被重新构建。伤口愈合延迟或失败是皮肤治疗的一个主要问题，因为它阻止了受伤组织的正常结构和功能的恢复，导致溃疡或其他皮肤异常。人永生化角质形成细胞（HaCAT）是一种自发永生化的人角质形成细胞系，能够分泌许多刺激皮肤细胞增殖、 rejuvenation 和再生的生物活性化学物质（secretome）。在这项研究中，HaCaT 分泌被聚（乳酸-共-羟基乙酸）（PLGA）和木薯淀粉等聚酯包裹，以最大限度地发挥其潜力。根据 HaCaT 分泌的估计机制，所有处理都在永生化真皮成纤维细胞系上进行，这是一种伤口愈合模型。HaCaT 分泌和木薯淀粉的包封增强了细胞增殖、迁移和抗衰老的效果。另一方面，活性氧（ROS）的水平降低，激活了永生化真皮成纤维细胞中的抗氧化剂。HaCaT 分泌以剂量依赖性方式诱导抗氧化相关基因的表达，包括 SOD、CAT 和 GPX。六种细胞因子，包括 CCL2 和 MCP-1，影响培养的 HaCAT 细胞中的免疫调节和炎症过程。用木薯淀粉包封的 HaCaT 分泌可以通过增加抗氧化剂来减少 ROS 的积累，从而刺激伤口愈合。因此，HaCaT 分泌物可能有机会在化妆品行业开发用于预防和治疗伤口的成分。
