种子提取物对创伤感染分离菌的抗菌作用研究。

A Study of Antibacterial Effect of Seed Extracts on Bacterial Isolates from Cases of Wound Infection.

机构信息

Department of Microbiology, Kasturba Medical College, Mangalore, Karnataka, India.

Department of Microbiology, Manipal Academy of Higher Education, Manipal, Karnataka, India.

出版信息

Infect Disord Drug Targets. 2023;23(5):e030423215400. doi: 10.2174/1871526523666230403095441.

DOI:10.2174/1871526523666230403095441

PMID:37016531

Abstract

BACKGROUND

With an increasing trend of pathogenic bacteria developing resistance to the existing drugs, there is a need for newer therapeutic measures. Nigella sativa seeds and oil have been used for decades as Ayurveda, Unani Tibb and other forms of traditional medicine for various disorders. Thymoquinone is one of the active components of the N. sativa seeds.

OBJECTIVE

The present study determines the antibacterial effect of crude methanolic extract N. sativa seeds and thymoquinone against bacteria causing wound infection.

METHODS

Samples obtained from cases of wound infection received at a Microbiology laboratory attached to a tertiary care hospital over a period of six months were included in the study. The antibacterial effect of crude methanolic extract of N. sativa seeds was determined by the Punch Well method. The minimum inhibitory concentration (MIC) of thymoquinone against bacteria isolated from cases of wound infection was determined by the Micro Broth Dilution technique.

RESULTS

A total of 60 isolates were collected from 60 samples of wound infection. By the Punch Well method, Staphylococcus aureus showed varying zones of inhibition whereas all gram-negative bacilli and Enterococcus faecalis did not show any zone of inhibition. Thymoquinone showed good antibacterial activity against S. aureus with MIC values ranging from 2-8μg/ml for most of the isolates. Uniformly, MIC of thymoquinone against all gram-negative bacilli and E. faecalis was >128μg/ml, p<0.001. It was found that methicillin-resistant S. aureus (MRSA) isolates showed higher MIC than methicillin sensitive S. aureus (MSSA) isolates p<0.05.

CONCLUSION

Antibacterial activity of thymoquinone was very good against S. aureus but showed limited activity against Enterobacteriaceae members and E. faecalis isolated from patients with wound infection. Thymoquinone may be considered a potential antibacterial agent against wound infection caused by S. aureus.

摘要

背景

随着致病菌对现有药物耐药性的不断增加，需要寻找新的治疗方法。黑种草子和油已被用于治疗各种疾病数十年，包括在阿育吠陀、顺势疗法和其他传统医学中。蒂莫昆酮是黑种草子的一种活性成分。

目的

本研究旨在确定黑种草子粗甲醇提取物和蒂莫昆酮对引起伤口感染的细菌的抗菌作用。

方法

将在一家三级护理医院附属微生物实验室就诊的 6 个月内的伤口感染病例的样本纳入研究。采用打孔法测定黑种草子粗甲醇提取物的抗菌作用。采用微量肉汤稀释法测定从伤口感染病例中分离的细菌对蒂莫昆酮的最小抑菌浓度（MIC）。

结果

从 60 份伤口感染样本中总共收集了 60 株分离株。通过打孔法，金黄色葡萄球菌表现出不同的抑菌环，而所有革兰氏阴性杆菌和粪肠球菌均未表现出任何抑菌环。蒂莫昆酮对金黄色葡萄球菌表现出良好的抗菌活性，大多数分离株的 MIC 值范围为 2-8μg/ml。同样，所有革兰氏阴性杆菌和粪肠球菌对蒂莫昆酮的 MIC 值均>128μg/ml，p<0.001。研究发现，耐甲氧西林金黄色葡萄球菌（MRSA）分离株的 MIC 值高于甲氧西林敏感金黄色葡萄球菌（MSSA）分离株，p<0.05。