黑种草对耐甲氧西林金黄色葡萄球菌临床分离株的抗菌活性。
Anti bacterial activity of Nigella sativa against clinical isolates of methicillin resistant Staphylococcus aureus.
作者信息
Hannan Abdul, Saleem Sidrah, Chaudhary Saadia, Barkaat Muhammad, Arshad Muhammad Usman
机构信息
Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
出版信息
J Ayub Med Coll Abbottabad. 2008 Jul-Sep;20(3):72-4.
BACKGROUND
Methicillin resistant Staphylococcus aureus (MRSA) continues to be one of the commonest pathogens encountered in clinical as well as laboratory practice. It has become a major health problem worldwide. Newer antimicrobials/agents are urgently needed to combat this problem MRSA resistance to various anti-staphylococcal agents. In the back-drop of this difficult situation Nigella sativa commonly known as black seed (ethanolic extract) was aimed at to evaluate if it had any anti-staphylococcal activity.
METHODS
The extract was prepared by reflux extraction method. Disc diffusion and in agar dilution methods were performed to assess the antibacterial activity. Staphylococcus aureus ATCC 25923 was used as the standard reference strain.
RESULTS
All tested strains of MRSA were sensitive to N. sativa extract at a concentration of 4 mg/disc while the extract had an MIC range of 0.2-0.5 mg/ml.
CONCLUSION
The results indicated that N. sativa has inhibitory effect on MRSA. This finding warrants necessity of further investigation of this product of folk medicine.
背景
耐甲氧西林金黄色葡萄球菌（MRSA）仍然是临床和实验室实践中最常见的病原体之一。它已成为全球主要的健康问题。迫切需要新型抗菌药物来应对MRSA对各种抗葡萄球菌药物产生耐药性这一问题。在这种严峻形势下，旨在评估通常被称为黑种草籽（乙醇提取物）是否具有抗葡萄球菌活性。
方法
通过回流提取法制备提取物。采用纸片扩散法和琼脂稀释法评估抗菌活性。金黄色葡萄球菌ATCC 25923用作标准参考菌株。
结果
所有测试的MRSA菌株在浓度为4mg/纸片时对黑种草籽提取物敏感，而该提取物的最低抑菌浓度范围为0.2 - 0.5mg/ml。
结论
结果表明黑种草籽对MRSA有抑制作用。这一发现证明有必要对这种民间药物产品进行进一步研究。
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