Hobson B M, Wide L
J Reprod Fertil. 1986 Mar;76(2):637-44. doi: 10.1530/jrf.0.0760637.
Chorionic gonadotrophin activity in extracts of the term placenta of a dolphin, a sea lion and a grey seal was measured by its effectiveness in increasing uterine weight in the mouse and by solid-phase RIA using hCG as immunogen and labelled antigen. Bioreactive (B) gonadotrophin was found in these placentae and, compared to the human term placenta, the concentration of CG in the dolphin was higher, in the sea lion similar and in the grey seal lower. The biological activity in each species was neutralized with a rabbit anti-hCG serum. All placental extracts contained material active in the hCG immunoassay (I). The ratio B/I was significantly higher for the CG in the placental extracts of the marine mammals compared with that of the human term placenta. Results of in-vivo bioassay, RIA, electrophoretic and gel-chromatographic studies indicate structural similarities between CG in the placentae of the marine mammals and human CG.
通过测量海豚、海狮和灰海豹足月胎盘提取物在增加小鼠子宫重量方面的有效性，以及使用人绒毛膜促性腺激素（hCG）作为免疫原和标记抗原的固相放射免疫分析（RIA），来测定其中绒毛膜促性腺激素的活性。在这些胎盘中发现了生物活性（B）促性腺激素，与人类足月胎盘相比，海豚胎盘中CG的浓度更高，海狮胎盘中的浓度相似，而灰海豹胎盘中的浓度更低。每种物种中的生物活性都用兔抗hCG血清进行了中和。所有胎盘提取物在hCG免疫分析（I）中都含有活性物质。与人类足月胎盘相比，海洋哺乳动物胎盘提取物中CG的B/I比值显著更高。体内生物测定、RIA、电泳和凝胶色谱研究的结果表明，海洋哺乳动物胎盘的CG与人类CG在结构上具有相似性。
