Wide L, Hobson B
Ups J Med Sci. 1978;83(1):1-6. doi: 10.3109/03009737809179104.
Acetone-ether extracts of rat, mouse and hamster placentae were fractionated by molecular sieve chromatography on Sephadex G-200. The fractions were tested for immunoreactivity in human chorionic gonadotropin (hCG), hCG-beta-subunit and alpha-subunit radio-immunoassay systems. The elution profiles were compared with those of similar chromatographic studies of a human placental extract and of purified preparations of hCG and its subunits. The results indicate that rodent placentae have a chorionic gonadotropin and that this hormone in the rat, mouse and hamster is structurally similar to hCG with its alpha- and beta-subunits. Extracts of rat and hamster placentae had a gonadotropic activity similar in concentration to that found in normal human placentae at term. Until now, it has been difficult to find an animal model for studying how the production of chorionic gonadotropin is regulated. Our results suggest that rodents may be suitable for such an investigation.
用葡聚糖凝胶G - 200对大鼠、小鼠和仓鼠胎盘的丙酮 - 乙醚提取物进行分子筛层析分级分离。在人绒毛膜促性腺激素（hCG）、hCG-β亚基和α亚基放射免疫分析系统中检测这些级分的免疫反应性。将洗脱图谱与对人胎盘提取物以及hCG及其亚基纯化制剂进行的类似色谱研究的图谱进行比较。结果表明啮齿动物胎盘含有绒毛膜促性腺激素，并且大鼠、小鼠和仓鼠体内的这种激素在结构上与hCG及其α和β亚基相似。大鼠和仓鼠胎盘提取物的促性腺活性在浓度上与足月正常人胎盘的促性腺活性相似。到目前为止，很难找到一个研究绒毛膜促性腺激素产生如何调控的动物模型。我们的结果表明啮齿动物可能适合进行这样的研究。
