Adams N R
J Reprod Fertil. 1986 Mar;76(2):727-33. doi: 10.1530/jrf.0.0760727.
This study examined the effects of prolonged exposure to oestradiol-17 beta on the morphology of the cervix of the ewe. Merino ewe lambs were implanted subcutaneously with 3 Silastic capsules which released a total of approximately 300 micrograms oestradiol-17 beta per day. After exposure for 200 days the uterus was more markedly bicornuate, and the cervix was broader and softer, than in controls. The cervical folds were shorter and contained many stromal cells. The amount of lamina propria under the folds was increased and altered so that it contained tubular glands and more stromal cells. The endocervix thus came to resemble endometrium. This appearance developed within 80 days of exposure, and remained for at least 170 days after implant removal. In a second experiment, mature multiparous Merino ewes were ovariectomized and implanted with 1, 2 or 4 similar oestradiol capsules for 140 days. Similar features developed in these ewes, and the degree of change was almost as great with 1 implant as with 4. Changes of a similar nature can be produced in other species by oestrogen given during organogenesis but not during adult life. The changes indicate that the ewe has an ability to display a degree of morphogenic response to oestradiol during adult life.
本研究检测了长期暴露于17β-雌二醇对母羊子宫颈形态的影响。将3个硅橡胶胶囊皮下植入美利奴母羊羔羊体内，这些胶囊每天总共释放约300微克17β-雌二醇。暴露200天后，与对照组相比，子宫的双角形态更明显，子宫颈更宽且更柔软。子宫颈皱襞更短，含有许多基质细胞。皱襞下方的固有层数量增加且发生改变，使其含有管状腺体和更多基质细胞。因此，子宫颈内膜开始类似于子宫内膜。这种外观在暴露80天内形成，并在取出植入物后至少持续170天。在第二个实验中，对成熟的经产美利奴母羊进行卵巢切除，并植入1个、2个或4个类似的雌二醇胶囊，持续140天。这些母羊也出现了类似的特征，1个植入物产生的变化程度几乎与4个植入物相同。在器官发生期间而非成年期给予雌激素，可在其他物种中产生类似性质的变化。这些变化表明，母羊在成年期具有对雌二醇表现出一定程度形态发生反应的能力。
