Zhao Yuyan, Jiang Xu, Zhou Shihuan, Tian Jin, Yang Piao, Chen Yanli, Zhang Quan, Xu Xianlin, Chen Yongzheng, Yang Jiawei
Department of Biochemistry, School of Preclinical Medicine, Zunyi Medical University, China.
Key Laboratory of Biocatalysis & Chiral Drug Synthesis of Guizhou Province, School of Pharmacy, Zunyi Medical University, China.
Org Biomol Chem. 2023 Apr 26;21(16):3417-3422. doi: 10.1039/d3ob00402c.
Optically pure sulfoxides are noteworthy compounds that find wide applications in various industrial fields. Here, we report a methionine sulfoxide reductase B (MsrB) homologue that exhibits high enantioselectivity and broad substrate scope for the kinetic resolution of racemic () sulfoxides. This MsrB homologue, named MsrB, was identified from sp. 103DPR2 and showed good activity together with enantioselectivity towards a series of aromatic, heteroaromatic, alkyl and thioalkyl sulfoxides. Chiral sulfoxides in the configuration were prepared in approximately 50% yield and 92-99% enantiomeric excess through kinetic resolution at an initial substrate concentration of up to 90 mM (11.2 g L). This study presents an efficient route for the enzymatic preparation of ()-sulfoxides through kinetic resolution.
光学纯的亚砜是值得关注的化合物，在各种工业领域有广泛应用。在此，我们报道了一种甲硫氨酸亚砜还原酶B（MsrB）同源物，它对消旋（）亚砜的动力学拆分表现出高对映选择性和广泛的底物范围。这种MsrB同源物，命名为MsrB，是从sp. 103DPR2中鉴定出来的，对一系列芳香族、杂芳香族、烷基和硫代烷基亚砜表现出良好的活性和对映选择性。通过在初始底物浓度高达90 mM（11.2 g L）下进行动力学拆分，以约50%的产率和92 - 99%的对映体过量制备了构型为的手性亚砜。本研究提出了一种通过动力学拆分酶法制备（） - 亚砜的有效途径。
