Burton-Wurster N, Butler M, Harter S, Colombo C, Quintavalla J, Swartzendurber D, Arsenis C, Lust G
J Rheumatol. 1986 Feb;13(1):175-82.
Fibronectin content was determined in articular cartilage in a spontaneous dog model and in a meniscectomy rabbit model of osteoarthritis. Determination of the fibronectin content of urea extracts of articular cartilage by an enzyme linked immunosorbent assay (ELISA) disclosed that degenerated cartilage contained from 10- to 40-fold more fibronectin than normal cartilage. The finding that cartilage fibronectin content was increased in both animal models suggests that elevated cartilage fibronectin content is a general feature of the osteoarthritic process. Immunoperoxidase studies disclosed that fibronectin was distributed throughout the matrix in hyaluronidase treated normal and osteoarthritic cartilage from both animal models, but quantitative differences in fibronectin were not observed by these techniques.
在自发性犬模型和半月板切除兔骨关节炎模型的关节软骨中测定纤连蛋白含量。通过酶联免疫吸附测定法（ELISA）测定关节软骨尿素提取物中的纤连蛋白含量，结果显示退变软骨中的纤连蛋白含量比正常软骨高10至40倍。在两种动物模型中软骨纤连蛋白含量均增加这一发现表明，软骨纤连蛋白含量升高是骨关节炎进程的一个普遍特征。免疫过氧化物酶研究表明，在两种动物模型经透明质酸酶处理的正常和骨关节炎软骨中，纤连蛋白分布于整个基质中，但这些技术未观察到纤连蛋白的定量差异。
