Brown R A, Jones K L
Department of Experimental Pathology, University of London, Stanmore, Middlesex, UK.
J Rheumatol. 1990 Jan;17(1):65-72.
Fibronectin was extracted from cartilage under denaturing conditions at concentrations of 1.5 and 15 micrograms/g wet weight for normal and osteoarthritic cartilage, respectively (i.e., there was a 10-fold increase in osteoarthritic cartilage). Heterogeneity on ion exchange and gelatin affinity chromatography and on agarose electrophoresis was consistent with the suggestion that it interacts strongly with elements of proteoglycans. Aggregation of cartilage proteoglycan by addition of hyaluronic acid also aggregated the endogenous fibronectin. Synthesis of fibronectin was identified in explants of normal cartilage by immunoprecipitation and electrophoresis. We conclude that cartilage fibronectin is a normal component of human cartilage and a normal product of mature chondrocytes.
在变性条件下，分别从正常软骨和骨关节炎软骨中提取纤连蛋白，提取浓度分别为每克湿重1.5微克和15微克（即骨关节炎软骨中的提取浓度增加了10倍）。离子交换、明胶亲和层析以及琼脂糖电泳显示的异质性与纤连蛋白与蛋白聚糖成分强烈相互作用的观点一致。添加透明质酸使软骨蛋白聚糖聚集，同时也使内源性纤连蛋白聚集。通过免疫沉淀和电泳在正常软骨外植体中鉴定出纤连蛋白的合成。我们得出结论，软骨纤连蛋白是人类软骨的正常成分，也是成熟软骨细胞的正常产物。
