Department of Oncology, The First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China.
Department of outpatient, The First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China.
Cancer Med. 2023 May;12(9):10791-10803. doi: 10.1002/cam4.5811. Epub 2023 Apr 5.
The underlying mechanisms of hepatocellular carcinoma (HCC) have not been fully investigated, and effective biomarkers for HCC are still needed to be explored. Therefore, our study sought to thoroughly examine the clinical significance and biological functions of the ribosomal protein L32 (RPL32) in HCC by coupling bioinformatic methods with experimental analysis.
To determine the clinical significance of RPL32, bioinformatic analyses were performed to examine RPL32 expression in HCC patient samples and to correlate RPL32 expression and HCC patient survival rates, genetic alterations, and immune cell infiltration. Cell counting kit-8 assays, colony formation, flow cytometry, and transwell assays were performed to examine the effects of RPL32 on HCC cell proliferation, apoptosis, migration, and invasion in HCC cell lines (SMMC-7721 and SK-HEP-1) where RPL32 was silenced using small interfering ribonucleic acid.
In the current study, we show that RPL32 was highly expressed in HCC samples. Moreover, high levels of RPL32 were associated with unfavorable outcomes in patients with HCC. Promoter methylation and copy number variation of RPL32 were associated with RPL32 mRNA expression. Results from the RPL32 silencing experiments indicated that the proliferation, apoptosis, migration, and invasion of SMMC-7721 and SK-HEP-1 cells were attenuated upon RPL32 depletion.
RPL32 correlates with a favorable prognosis in patients with HCC and promotes the survival, migration, and invasion of HCC cells.
肝细胞癌（HCC）的潜在机制尚未被充分研究，仍需要探索用于 HCC 的有效生物标志物。因此，我们通过将生物信息学方法与实验分析相结合，研究了核糖体蛋白 L32（RPL32）在 HCC 中的临床意义和生物学功能。
为了确定 RPL32 的临床意义，我们进行了生物信息学分析，以检查 HCC 患者样本中的 RPL32 表达，并将 RPL32 表达与 HCC 患者的生存率、遗传改变和免疫细胞浸润相关联。通过细胞计数试剂盒-8 测定法、集落形成实验、流式细胞术和 Transwell 测定法，在沉默 RPL32 的 HCC 细胞系（SMMC-7721 和 SK-HEP-1）中检查了 RPL32 对 HCC 细胞增殖、凋亡、迁移和侵袭的影响，这些细胞系使用小干扰核糖核酸沉默 RPL32。
在本研究中，我们表明 RPL32 在 HCC 样本中高度表达。此外，高水平的 RPL32与 HCC 患者的不良预后相关。RPL32 的启动子甲基化和拷贝数变异与 RPL32 mRNA 表达相关。RPL32 沉默实验的结果表明，RPL32 耗尽可减弱 SMMC-7721 和 SK-HEP-1 细胞的增殖、凋亡、迁移和侵袭。
RPL32 与 HCC 患者的良好预后相关，并促进 HCC 细胞的存活、迁移和侵袭。
