da Silva Karine Letícia, Favoreto Michael Willian, Centenaro Gabrielle Gomes, Bernardi Laís Giacomini, Borges Christiane Philippini Ferreira, Reis Alessandra, Loguercio Alessandro D
Department of Restorative Dentistry, State University of Ponta Grossa, Rua Carlos Cavalcanti, 4748, Bloco M - Uvaranas, Ponta Grossa, Paraná, Brazil.
Department of Chemistry, State University of Ponta Grossa, Parana, Brazil.
Clin Oral Investig. 2023 Jul;27(7):3663-3671. doi: 10.1007/s00784-023-04980-w. Epub 2023 Apr 5.
This in vitro study aims to evaluate of hydrogen peroxide (HP) diffusion into the pulp chamber, bleaching efficacy (BE), and pH stability (pH) of single-application high concentrated in-office bleaching gels.
Eighty-eight healthy premolars were randomly into eleven groups (n = 8) according to the in-office dental bleaching: DSP White Clinic 35% calcium (DW), Nano White 35% (NW), Opalescence XTra Boost 40% (OB), Pola Office + 37.5% (PO), Potenza Bianco Pro SS 38% (PB), Total Blanc 35% (TB), Total Blanc One-Step 35% (TO), Whiteness Automixx 35% (WA), Whiteness Automixx Plus 35% (WP), and Whiteness HP Blue 35% (WB). A group not exposed to bleaching agents was the control group (CG). All bleaching agents were applied in one session with a single application. After the bleaching procedure, the concentration of HP diffusion (μg/mL) into the pulp chamber was assessed using UV-Vis spectrophotometry. The BE (ΔE and ΔE) was evaluated before and 1 week after the bleaching procedure using a digital spectrophotometer. The pH of each bleaching gel was evaluated by digital pHmeter. The one-way ANOVA and Tukey's was used for a statistical analysis (α = 0.05).
The concentration of HP diffusion into the pulp chamber was higher in all in-office bleaching gels when compared to CG (p < 0.0000001). However, there are a significant difference between them (p = 0.0001). A significant BE was observed in all in-office bleaching gels (p < 0.0001 for ΔE and ΔE), with a significant difference between them (p < 0.0001). PO, OB, TB, WP, and WB showed a higher BE when compared to DW, PB, and WA (p < 0.0001). Most bleaching gels were slightly acidic or alkaline during the total application time, while DW, PB, TB, and WA showed a high acidic behavior after 30 min of application.
A single application was able to produce a bleaching efficacy. However, usually, gels with slightly acidic or alkaline pH during the application time reduces the HP diffusion into the pulp chamber.
The single-application of bleaching gels with slightly acidic or alkaline and stable pH decreased the penetration of hydrogen peroxide into the pulp chamber in in-office bleaching and maintained the bleaching efficacy.
本体外研究旨在评估过氧化氢（HP）向髓腔的扩散情况、单次应用高浓度诊室漂白凝胶的漂白效果（BE）和pH稳定性（pH）。
根据诊室牙齿漂白情况，将88颗健康前磨牙随机分为11组（n = 8）：DSP White Clinic 35%钙（DW）、Nano White 35%（NW）、Opalescence XTra Boost 40%（OB）、Pola Office + 37.5%（PO）、Potenza Bianco Pro SS 38%（PB）、Total Blanc 35%（TB）、Total Blanc One-Step 35%（TO）、Whiteness Automixx 35%（WA）、Whiteness Automixx Plus 35%（WP）和Whiteness HP Blue 35%（WB）。未暴露于漂白剂的一组为对照组（CG）。所有漂白剂均单次应用于一个疗程。漂白程序后，使用紫外可见分光光度法评估HP向髓腔扩散的浓度（μg/mL）。使用数字分光光度计在漂白程序前和漂白后1周评估BE（ΔE和ΔE）。通过数字pH计评估每种漂白凝胶的pH。采用单因素方差分析和Tukey检验进行统计分析（α = 0.05）。
与CG相比，所有诊室漂白凝胶中HP向髓腔扩散的浓度均较高（p < 0.0000001）。然而，它们之间存在显著差异（p = 0.0001）。在所有诊室漂白凝胶中均观察到显著的BE（ΔE和ΔE的p < 0.0001），且它们之间存在显著差异（p < 0.0001）。与DW、PB和WA相比，PO、OB、TB、WP和WB显示出更高的BE（p < 0.0001）。在整个应用时间内，大多数漂白凝胶呈微酸性或碱性，而DW、PB、TB和WA在应用30分钟后表现出高酸性行为。
单次应用能够产生漂白效果。然而，通常在应用期间pH呈微酸性或碱性的凝胶会减少HP向髓腔的扩散。
单次应用pH呈微酸性或碱性且稳定的漂白凝胶可降低诊室漂白中过氧化氢向髓腔的渗透，并维持漂白效果。
