Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
J Biol Inorg Chem. 2023 Jun;28(4):379-391. doi: 10.1007/s00775-023-01996-1. Epub 2023 Apr 5.
Hydroxytyrosol, one of the most powerful natural antioxidants, exhibits certificated benefits for human health. In this study, a biomimetic approach to synthesize hydroxytyrosol from the hydroxylation of tyrosol was established. EDTA-Fe coordination complex served as an active center to simulate tyrosine hydroxylase. HO and ascorbic acid were used as oxygen donor and hydrogen donor, respectively. Hydroxy radical and singlet oxygen contributed to active species. The biomimetic system displayed analogous component, structure, and activity with TyrH. Hydroxytyrosol titer of 21.59 mM, and productivity of 9985.92 mg·L·h was achieved with 100 mM tyrosol as substrate. The proposed approach provided efficient and convenient route to quickly produce high amount of hydroxytyrosol.
羟基酪醇是一种最强大的天然抗氧化剂，已被证实对人体健康有益。在本研究中，建立了一种从酪氨酸羟化合成羟基酪醇的仿生方法。EDTA-Fe 配合物作为活性中心模拟酪氨酸羟化酶。HO 和抗坏血酸分别作为氧供体和氢供体。羟自由基和单线态氧作为活性物质。仿生体系与 TyrH 具有相似的组成、结构和活性。以 100mM 酪氨酸为底物，可获得 21.59mM 的羟基酪醇浓度和 9985.92mg·L·h 的产率。该方法为快速大量生产羟基酪醇提供了有效便捷的途径。
