Allouche N, Damak M, Ellouz R, Sayadi S
Laboratoire des Bioprocédés, Centre de Biotechnologie de Sfax, 3038 Sfax, Tunisia.
Appl Environ Microbiol. 2004 Apr;70(4):2105-9. doi: 10.1128/AEM.70.4.2105-2109.2004.
For the first time, a soil bacterium, designated Pseudomonas aeruginosa, was isolated based on its ability to grow on tyrosol as a sole source of carbon and energy. During growth on tyrosol, this strain was capable of promoting the formation of a significant amount of hydroxytyrosol and trace quantities of parahydroxyphenyl acetic acid and 3,4-dihydroxyphenyl acetic acid. The products were confirmed by high-performance liquid chromatography and gas chromatography-mass spectrometry analyses. Using an optimized tyrosol concentration of 2 g liter(-1), the maximal hydroxytyrosol yield (80%) was achieved after a 7-h reaction in a growth experiment. To enhance the formation of hydroxytyrosol and prevent its degradation, a resting-cell method using P. aeruginosa was performed. The growth state of the culture utilized for biomass production, the carbon source on which the biomass was grown, the concentration of the biomass, and the amount of tyrosol that was treated were optimized. The optimal yield of hydroxytyrosol (96%) was obtained after a 7-h reaction using 4 g of tyrosol liter(-1) and 5 g of cells liter(-1) pregrown on tyrosol and harvested at the end of the exponential phase. This proposed procedure is an alternative approach to obtain hydroxytyrosol in an environmentally friendly way. In addition, the reaction is easy to perform and can be adapted to a bioreactor for industrial purposes.
首次分离出一种土壤细菌，命名为铜绿假单胞菌，它是根据其能够以酪醇作为唯一碳源和能源生长的能力分离出来的。在以酪醇为碳源生长的过程中，该菌株能够促进大量羟基酪醇的形成，并产生微量的对羟基苯乙酸和3,4 -二羟基苯乙酸。这些产物通过高效液相色谱和气相色谱 - 质谱分析得到了确认。在生长实验中，使用优化后的酪醇浓度2 g·L⁻¹，7小时反应后可实现最大羟基酪醇产率（80%）。为了提高羟基酪醇的形成并防止其降解，采用了铜绿假单胞菌的静息细胞法。对用于生物质生产的培养物的生长状态、生物质生长所使用的碳源、生物质的浓度以及处理的酪醇量进行了优化。使用4 g·L⁻¹酪醇和5 g·L⁻¹在酪醇上预生长并在指数期末收获的细胞，7小时反应后可获得羟基酪醇的最佳产率（96%）。该方法是一种以环境友好方式获得羟基酪醇的替代方法。此外，该反应易于进行，并且可以适用于工业用途的生物反应器。
