Binaglia L, Roberti R, Freysz L, Arienti G, Corazzi L, Porcellati G
J Membr Biol. 1986;90(1):29-35. doi: 10.1007/BF01869683.
The compartmentation of the phosphatidylethanolamine newly synthesized in brain microsomes in vitro either by base exchange or net synthesis has been studied, using difluorodinitrobenzene as a chemical probe. The experimental results demonstrate that in rat brain microsomes the phosphatidylethanolamine molecules synthesized by base exchange and the bulk membrane lipid belong to different pools. Ca2+ bound to microsomes seems to be involved in the maintenance of the compartmentation of phosphatidylethanolamine. In the presence of Ca2+ the newly synthesized phosphatidylethanolamine molecules react with difluorodinitrobenzene as though they are organized in clusters. After biosynthesis in vivo or in vitro through the cytidine pathway, the compartmentation of the newly formed phosphatidylethanolamine appears less marked than after the synthesis through base exchange.
利用二氟二硝基苯作为化学探针，对体外脑微粒体中新合成的磷脂酰乙醇胺通过碱基交换或净合成进行区室化的情况进行了研究。实验结果表明，在大鼠脑微粒体中，通过碱基交换合成的磷脂酰乙醇胺分子与大部分膜脂属于不同的池。与微粒体结合的Ca2+似乎参与了磷脂酰乙醇胺区室化的维持。在Ca2+存在的情况下，新合成的磷脂酰乙醇胺分子与二氟二硝基苯反应，就好像它们以簇的形式组织起来一样。通过胞苷途径在体内或体外进行生物合成后，新形成的磷脂酰乙醇胺的区室化程度似乎不如通过碱基交换合成后那么明显。
