大鼠脑微粒体中通过碱基交换反应形成的膜磷脂酰乙醇胺的区室化。

Compartmentation of membrane phosphatidylethanolamine formed by base-exchange reaction in rat brain microsomes.

作者信息

Corazzi L, Binaglia L, Roberti R, Freysz L, Arienti G, Porcellati G

出版信息

Biochim Biophys Acta. 1983 Apr 21;730(1):104-10. doi: 10.1016/0005-2736(83)90322-x.

Abstract

The compartmentation of membrane phosphatidylethanolamine (PE) formed by base-exchange reaction in rat brain microsomal vesicles has been investigated. After labelling membrane PE by base-exchange in vitro, microsomal vesicles were treated with trinitrobenzenesulfonic acid (TNBS). The amount of membrane PE reacting with TNBS depends on the duration and the temperature of the reaction as well as on the TNBS concentration. It was found that almost all of the labelled PE molecules, but only about 24% of membrane PE, were accessible to TNBS, under very mild reaction conditions. It is concluded that PE labelled by base-exchange is completely localized in the cytoplasmic leaflet of microsomal vesicles.

摘要

已对大鼠脑微粒体囊泡中通过碱基交换反应形成的膜磷脂酰乙醇胺（PE）的区室化进行了研究。在体外通过碱基交换标记膜PE后，用三硝基苯磺酸（TNBS）处理微粒体囊泡。与TNBS反应的膜PE量取决于反应的持续时间、温度以及TNBS浓度。发现在非常温和的反应条件下，几乎所有标记的PE分子，但只有约24%的膜PE可与TNBS接触。得出的结论是，通过碱基交换标记的PE完全定位于微粒体囊泡的细胞质小叶中。