Xia Zhiwen, Zhang Xin, Yao Jingyuan, Liu Zihao, Jin Yulong, Yin Huabing, Wang Pu, Wang Xiu-Hong
Laboratory for Biomedical Photonics, Institute of Laser Engineering, Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing 100124, China.
Laboratory for Advanced Laser Technology and Applications, Institute of Laser Engineering, Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing 100124, China.
ACS Sens. 2023 Apr 28;8(4):1799-1809. doi: 10.1021/acssensors.3c00131. Epub 2023 Apr 5.
Microstructured optical fibers (MOFs) provide solutions for breaking through the bottlenecks in areas of high-power transmission and high-efficiency optical waveguides. Other than transporting light waves, MOFs can synergistically combine microfluidics and optics in a single fiber with an unprecedented light path length not readily achievable by planar optofluidic configurations. Here, we demonstrate that hollow-core anti-resonant optical fibers (HcARFs) can significantly enhance Raman scattering by over three orders of magnitude (EF ≈ 5000) compared with a planar setup, due to the joint mechanisms of strong light-matter interaction in the fiber core and the cumulative effect of the fiber. The giant enhancement enables us to develop the first optical fiber sensor to achieve single cancer exosome detection via a sandwich-structured strategy. This enables a multiplexed analysis of surface proteins of exosome samples, potentially allowing an accurate identification of the cellular origin of exosomes for cancer diagnosis. Our findings could expand the applications of HcARF in many exciting areas beyond the waveguide.
微结构光纤（MOF）为突破高功率传输和高效光波导领域的瓶颈提供了解决方案。除了传输光波外，MOF还能将微流体和光学在单根光纤中协同结合，其光程长度前所未有的长，这是平面光流体配置难以实现的。在此，我们证明，与平面装置相比，空心反谐振光纤（HcARF）由于光纤芯中强光 - 物质相互作用的联合机制以及光纤的累积效应，可将拉曼散射显著增强三个数量级以上（增强因子约为5000）。这种巨大的增强使我们能够开发出首个通过夹心结构策略实现单个癌症外泌体检测的光纤传感器。这使得对外泌体样品的表面蛋白进行多重分析成为可能，从而有可能准确识别外泌体的细胞来源用于癌症诊断。我们的研究结果可能会将HcARF的应用扩展到光波导之外的许多令人兴奋的领域。
