School of Food Science and Technology, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
School of Food and Bioengineering, Xihua University, Chengdu 610039, China.
Int J Biol Macromol. 2023 Jun 15;240:124287. doi: 10.1016/j.ijbiomac.2023.124287. Epub 2023 Apr 3.
Developing high-strength hydrogels with biocompatibility and bone conductibility is still desirable for bone regeneration. The nanohydroxyapatite (nHA) was incorporated into a dopamine-modified gelatin (Gel-DA) hydrogel system to create a highly biomimetic native bone tissue microenvironment. In addition, to further increase the cross-linking density between nHA and Gel-DA, nHA was functionalized by mussel-inspired polydopamine (PDA). Compared with nHA, adding polydopamine functionalized nHA (PHA) increased the compressive strength of Gel-Da hydrogel from 449.54 ± 180.32 kPa to 611.18 ± 211.86 kPa without affecting its microstructure. Besides, the gelation time of Gel-DA hydrogels with PHA incorporation (GD-PHA) was controllable from 49.47 ± 7.93 to 88.11 ± 31.18 s, contributing to its injectable ability in clinical applications. In addition, the abundant phenolic hydroxyl group of PHA was beneficial to the cell adhesion and proliferation of Gel-DA hydrogels, leading to the excellent biocompatibility of Gel-PHA hydrogels. Notably, the GD-PHA hydrogels could accelerate the bone repair efficiency in the rat model of the femoral defect. In conclusion, our results suggest the Gel-PHA hydrogel with osteoconductivity, biocompatibility, and enhanced mechanical properties is a potential bone repair material.
开发具有生物相容性和骨传导性的高强度水凝胶仍然是骨再生的理想选择。将纳米羟基磷灰石（nHA）掺入多巴胺修饰的明胶（Gel-DA）水凝胶系统中，以创造高度仿生的天然骨组织微环境。此外，为了进一步提高 nHA 和 Gel-DA 之间的交联密度，通过贻贝启发的聚多巴胺（PDA）对 nHA 进行功能化。与 nHA 相比，添加聚多巴胺功能化 nHA（PHA）将 Gel-Da 水凝胶的压缩强度从 449.54±180.32kPa 提高到 611.18±211.86kPa，而不会影响其微观结构。此外，含有 PHA 的 Gel-DA 水凝胶的凝胶时间（GD-PHA）从 49.47±7.93s 可控至 88.11±31.18s，有助于其在临床应用中的可注射性。此外，PHA 丰富的酚羟基有利于 Gel-DA 水凝胶的细胞黏附和增殖，从而使 Gel-PHA 水凝胶具有优异的生物相容性。值得注意的是，GD-PHA 水凝胶可加速大鼠股骨缺损模型中的骨修复效率。总之，我们的结果表明，具有骨传导性、生物相容性和增强机械性能的 Gel-PHA 水凝胶是一种有潜力的骨修复材料。
