噬菌体T4 DNA的末端结构与包装机制
End structure and mechanism of packaging of bacteriophage T4 DNA.
作者信息
Kalinski A, Black L W
出版信息
J Virol. 1986 Jun;58(3):951-4. doi: 10.1128/JVI.58.3.951-954.1986.
Abstract
We analyzed by restriction enzyme digestion the end structure of T4 phage DNA by comparing mature, concatemeric, first-packaged, and incompletely packaged DNAs. The structure of mature DNA was also studied using 3' end labeling with terminal transferase. Our data support the hypothesis that T4 DNA packaging is not initiated at specific packaging initiation sequences on the concatemeric precursor (cos or pac site mechanisms) but by a different packaging mechanism.
摘要
我们通过限制性酶切消化，比较成熟的、串联体的、首次包装的和未完全包装的DNA，分析了T4噬菌体DNA的末端结构。还使用末端转移酶进行3'末端标记研究了成熟DNA的结构。我们的数据支持这样的假说，即T4 DNA包装不是在串联体前体上的特定包装起始序列（cos或pac位点机制）处起始，而是通过一种不同的包装机制。
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