Chung Jin-Yong, Ma Yongjie, Zhang Dingguo, Bickerton Hayden H, Stokes Eric, Patel Sweta B, Tse Hubert M, Feduska Joseph, Welner Rob S, Banerjee Ronadip R
Division of Endocrinology, Diabetes & Metabolism, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA.
Department of Pharmacology, the University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USA.
iScience. 2023 Mar 17;26(4):106439. doi: 10.1016/j.isci.2023.106439. eCollection 2023 Apr 21.
Pancreatic β-cell mass expands during pregnancy and regresses in the postpartum period in conjunction with dynamic metabolic demands on maternal glucose homeostasis. To understand transcriptional changes driving these adaptations in β-cells and other islet cell types, we performed single-cell RNA sequencing on islets from virgin, late gestation, and early postpartum mice. We identified transcriptional signatures unique to gestation and the postpartum in β-cells, including induction of the AP-1 transcription factor subunits and other genes involved in the immediate-early response (IEGs). In addition, we found pregnancy and postpartum-induced changes differed within each endocrine cell type, and in endothelial cells and antigen-presenting cells within islets. Together, our data reveal insights into cell type-specific transcriptional changes responsible for adaptations by islet cells to pregnancy and their resolution postpartum.
胰腺β细胞团在孕期会扩张，产后随着母体葡萄糖稳态的动态代谢需求而退化。为了解驱动β细胞和其他胰岛细胞类型发生这些适应性变化的转录变化，我们对未孕、妊娠晚期和产后早期小鼠的胰岛进行了单细胞RNA测序。我们鉴定出了β细胞中孕期和产后特有的转录特征，包括AP-1转录因子亚基和其他参与早期即刻反应（IEGs）的基因的诱导。此外，我们发现每种内分泌细胞类型以及胰岛内的内皮细胞和抗原呈递细胞中，妊娠和产后诱导的变化存在差异。总之，我们的数据揭示了胰岛细胞对妊娠及其产后恢复所做适应性变化的细胞类型特异性转录变化的见解。
