Wiggins R C, Glatfelter A, Kshirsagar B, Brukman J
Kidney Int. 1986 Feb;29(2):591-7. doi: 10.1038/ki.1986.39.
Procoagulant activity (PCA) in normal human urine was found to be sedimented by centrifugation at X 100,000g. Therefore, studies were done to identify the structures associated with the procoagulant activity. Transmission electron microscopy of the X 100,000g pellet revealed numerous membrane-bound vesicles as well as fibrous material. Filtration of normal urine through a 0.2-micron filter removed more than 90% of the procoagulant activity. Scanning electron microscopy of the filter surface revealed 0.1 to 1.1 micron particles and fibrous material. By centrifugation at pH 3 and 5 the fibrous material and particles were separated. The procoagulant activity remained with the particles in each case. The fibrous material was shown to be Tamm-Horsfall protein by SDS-PAGE and Western blotting using anti-Tamm-Horsfall protein serum. Purified Tamm-Horsfall protein itself was not procoagulant. Therefore, PCA in normal human urine is associated with particles 0.1 to 1.1 micron in diameter which appear to be lipid membranes in various arrangements.
正常人类尿液中的促凝活性（PCA）在100,000g离心时会沉淀。因此，开展了相关研究以鉴定与促凝活性相关的结构。对100,000g沉淀进行透射电子显微镜检查，发现有大量膜结合囊泡以及纤维状物质。用0.2微米滤器过滤正常尿液可去除90%以上的促凝活性。对滤器表面进行扫描电子显微镜检查，发现有0.1至1.1微米的颗粒和纤维状物质。通过在pH值为3和5时离心，可分离出纤维状物质和颗粒。在每种情况下，促凝活性都与颗粒在一起。通过SDS-PAGE和使用抗Tamm-Horsfall蛋白血清的蛋白质印迹法显示，纤维状物质为Tamm-Horsfall蛋白。纯化的Tamm-Horsfall蛋白本身没有促凝作用。因此，正常人类尿液中的PCA与直径为0.1至1.1微米的颗粒相关，这些颗粒似乎是呈各种排列的脂质膜。
