Wack R F, Hamlin R L
Lab Anim Sci. 1986 Apr;36(2):186-8.
Hearts isolated from healthy chickens were perfused with oxygenated Krebs-Henseleit buffer. Balloons were inserted into the left ventricle, filled with saline and attached to a pressure transducer for measuring left ventricular pressures. A bipolar electrogram was obtained between the atria and ventricles. Hearts so perfused manifested mechanical alternans for approximately 15 minutes, after which they sustained a regular sinus rhythm without mechanical alternans for at least 60 minutes. By infusing 0.1 ml increments of saline into the balloon located within the ventricle, Frank-Starling curves could be generated. This preparation has potential for use in pharmacologic evaluations.
从健康鸡身上分离出的心脏用含氧的克氏-亨氏缓冲液灌注。将气球插入左心室，充入盐水，并连接到压力传感器以测量左心室压力。在心房和心室之间获得双极心电图。如此灌注的心脏表现出约15分钟的机械交替，之后它们维持至少60分钟的规则窦性心律且无机械交替。通过向位于心室内的气球中逐次注入0.1毫升盐水，可以生成弗兰克-斯塔林曲线。这种制备方法有用于药理学评估的潜力。
