Centro de Investigação de Microrganismos, FAMERP, São José do Rio Preto, São Paulo, Brazil.
Laboratório de Pesquisa em Resistência e Virulência Bacteriana, FCFRP-USP, Ribeirão Preto, São Paulo, Brazil.
Diagn Microbiol Infect Dis. 2023 Jun;106(2):115932. doi: 10.1016/j.diagmicrobio.2023.115932. Epub 2023 Mar 4.
Carbapenemase-producing Klebsiella pneumoniae (CPKp) infections are important threats to pediatric populations. Thus, a retrospective study was conducted in a Brazilian reference pediatric hospital, and 26 CPKp isolates obtained from 23 patients were characterized. The affected population had important underlying diseases, reflecting previous hospitalization and antibiotic use. Most CPKp isolates were resistant to all antibiotic classes, and bla was the only carbapenemase-encoding gene. bla was common among the isolates, and modification or absence of the mgrB gene was the cause of polymyxin B resistance. Ten different sequence types were identified, and clonal complex 258 was prevalent. Alleles wzi50 and wzi64 were the most recurrent ones regarding K-locus type, with a remarkable contribution of the epidemic ST11/KL64 lineage as a colonizer. Our findings show that lineages associated with the pediatric population are similar to those found in adults, reinforcing the need for epidemiological surveillance to effectively implement prevention and control measures.
产碳青霉烯酶肺炎克雷伯菌（CPKp）感染对儿科人群构成重大威胁。因此，对巴西一家儿科参考医院进行了回顾性研究，对 23 名患者的 26 株 CPKp 分离株进行了特征描述。受影响人群存在重要的潜在疾病，反映了先前的住院和抗生素使用情况。大多数 CPKp 分离株对所有抗生素类别均具有耐药性，bla 是唯一编码碳青霉烯酶的基因。bla 在分离株中很常见，mgrB 基因的修饰或缺失是多粘菌素 B 耐药的原因。鉴定出 10 种不同的序列类型，258 号克隆复合体流行。wzi50 和 wzi64 等位基因是 K 基因座类型中最常见的两种，流行的 ST11/KL64 谱系作为定植者的贡献显著。我们的研究结果表明，与儿科人群相关的谱系与成人中发现的谱系相似，这强调了需要进行流行病学监测，以有效实施预防和控制措施。
