McGuire W L, Dressler L G, Sledge G W, Ramzy I, Ciocca D R
J Steroid Biochem. 1986 Jan;24(1):155-9. doi: 10.1016/0022-4731(86)90045-2.
The presence and distribution of a protein with a mol. wt of 24,000 (24K) was determined in endometrial biopsies from regularly cycling women and in women with endometrial carcinoma. This protein, of as yet unknown function and originally found in a breast cancer cell line, was detected by immunohistochemistry using a monoclonal antibody. In regularly cycling women, the 24K protein began to appear in the glandular epithelium during the late proliferative phase and decreased after ovulation. In contrast, in the superficial epithelium, the strongest immunostaining was observed during the secretory phase. Superficial epithelial cells expressed maximal 24K immunoreactivity around day 21 of the cycle and it was clearly seen in the bulbous projections of the apical cytoplasm. These results suggest that the 24K protein may be a marker for hormonal events in the endometrium during the menstrual cycle. In endometrial carcinoma, 24K was correlated with low tumor histologic grade, few mitotic figures, few nucleoli and a low degree of nuclear pleomorphism. These data suggest that 24K may be a potential marker of tumor differentiation.
在月经周期正常的女性及子宫内膜癌患者的子宫内膜活检组织中，对分子量为24,000（24K）的一种蛋白质的存在情况及分布进行了测定。这种功能尚不清楚、最初在一种乳腺癌细胞系中发现的蛋白质，通过使用单克隆抗体的免疫组织化学方法进行检测。在月经周期正常的女性中，24K蛋白质在增殖晚期开始出现在腺上皮中，并在排卵后减少。相比之下，在表层上皮中，在分泌期观察到最强的免疫染色。表层上皮细胞在月经周期的第21天左右表达最大的24K免疫反应性，并且在顶端细胞质的球状突起中清晰可见。这些结果表明，24K蛋白质可能是月经周期中子宫内膜激素变化的一个标志物。在子宫内膜癌中，24K与肿瘤组织学分级低、有丝分裂象少、核仁少及核异型性程度低相关。这些数据表明，24K可能是肿瘤分化的一个潜在标志物。
