用体外放射自显影术和计算机图像分析技术对大鼠脑中两种皮质酮受体位点进行解剖学定位。

Anatomical resolution of two types of corticosterone receptor sites in rat brain with in vitro autoradiography and computerized image analysis.

作者信息

Reul J M, de Kloet E R

出版信息

J Steroid Biochem. 1986 Jan;24(1):269-72. doi: 10.1016/0022-4731(86)90063-4.

Abstract

The rat brain contains two receptor systems for corticosterone (CORT): the glucocorticoid (GR) and corticosterone or mineralocorticoid-like (CR) receptor sites. We have studied the localization of these receptors by in vitro autoradiography and by in vitro cytosol binding assays in microdissected brain areas. In vitro autoradiography revealed that CR receptor sites are almost entirely restricted to the septal-hippocampal complex, whereas the presence of GR extends throughout the brain. Highest levels of GR are present in the lateral septum, hippocampal, cortical and thalamic regions and the paraventricular nucleus. In vitro determination of binding of 3H-labelled steroids to CR and GR in cytosol of "punched out" brain tissue revealed a similar neuroanatomical distribution as observed with the autoradiographic analysis. In addition, it was found that CORT binds to CR (KD approximately 0.5 nM) with 5-10-fold higher affinity than to GR (KD approximately 2.5-5 nM).

摘要

大鼠脑内存在两种皮质酮（CORT）受体系统：糖皮质激素（GR）受体位点和皮质酮或盐皮质激素样（CR）受体位点。我们通过体外放射自显影和对显微切割脑区进行体外胞浆结合试验，研究了这些受体的定位。体外放射自显影显示，CR受体位点几乎完全局限于隔区 - 海马复合体，而GR则遍布整个大脑。GR水平最高的区域是外侧隔区、海马、皮质、丘脑区域以及室旁核。对“打孔”脑组织胞浆中3H标记类固醇与CR和GR结合的体外测定显示，其神经解剖分布与放射自显影分析结果相似。此外，还发现CORT与CR结合（解离常数KD约为0.5 nM）的亲和力比与GR结合（解离常数KD约为2.5 - 5 nM）高5 - 10倍。