Verhage H G, Jaffe R C
J Steroid Biochem. 1986 Feb;24(2):587-90. doi: 10.1016/0022-4731(86)90124-x.
The purpose of this study was to determine the early effect of progesterone (P) on the estradiol (E2) and P receptor systems in cat uteri. Ovariectomized animals were treated with E2 for 7 days. Animals were then treated for up to 48 h with E2 and P, treated with P while being E2 withdrawn, or just E2 withdrawn. P treatment resulted in a significant decrease in P cytosol receptor (PcR) and a significant increase in P nuclear receptor (PnR) at all times included in this study when compared to levels measured in the E2-treated animal. E2 cytosol receptor (EcR) and E2 nuclear receptor (EnR) levels were significantly lower after 12 h of P treatment and remained so for the duration of this study. When EcR and EnR levels were compared after 48 h of P treatment in the presence or absence of E2, or after 48 h of E2 withdrawal, the loss of EnR following P treatment appeared to be independent of any changes in EcR levels or serum E2 levels, and only dependent on the presence of P. These results clearly illustrate that the chronic administration of P decreases the uterine concentration of its own receptor, and suggests that P decreases the E2 receptor system by a selective action within the nucleus which diminishes their ability to retain EnR.
本研究的目的是确定孕酮（P）对猫子宫中雌二醇（E2）及P受体系统的早期作用。对去卵巢动物给予E2治疗7天。然后，动物接受E2和P联合治疗长达48小时，在撤去E2的同时给予P治疗，或仅撤去E2。与E2治疗动物所测水平相比，在本研究涵盖的所有时间点，P治疗均导致P胞浆受体（PcR）显著降低，P核受体（PnR）显著增加。P治疗12小时后，E2胞浆受体（EcR）和E2核受体（EnR）水平显著降低，并在本研究期间一直保持较低水平。当比较在有或无E2情况下P治疗48小时后，或E2撤药48小时后的EcR和EnR水平时，P治疗后EnR的减少似乎与EcR水平或血清E2水平的任何变化无关，仅取决于P的存在。这些结果清楚地表明，长期给予P会降低其自身受体在子宫中的浓度，并提示P通过在细胞核内的选择性作用降低E2受体系统，从而削弱其保留EnR的能力。
