Saito-Nakano Yumiko, Umeki Yuko, Shimokawa Chikako, Kobayashi Koichi, Hashimoto Koichi, Takada Toshio, Makii Chinami, Hasebe Rie, Yoshida Yuri, Nakajima Riko, Kobayashi Seiki, Hisaeda Hajime
Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
IJID Reg. 2023 Feb 28;7:130-135. doi: 10.1016/j.ijregi.2023.02.007. eCollection 2023 Jun.
is the most prevalent sexually transmitted parasite worldwide. However, no surveillance system exists to monitor cases and drug resistance in Japan.
Cervical cytology vaginal swabs were collected from women with and without suspected symptoms of infection; these swabs were used for the detection of , human papillomavirus (HPV), and using specific polymerase chain reaction. Clinical isolates of were subjected to metronidazole susceptibility tests using the previously reported minimal lethal concentration (MLC) and newly established half-maximal inhibitory concentration (IC) values.
The prevalence of in the study population was 4.2% (5/119; 95% confidence interval [Cl], 1.5-9.7). Additionally, asymptomatic infection constituted 60% (3/5) of all cases of infection. All -positive patients were coinfected with HPV but not . Five clinical isolates showed metronidazole susceptibility, which was evaluated using MLC values. The quantitative IC values revealed that two of these clinical isolates exhibited a decreased metronidazole susceptibility.
This is the first study to demonstrate the prevalence of in Japanese women. The IC values of metronidazole against enabled the precise and quantitative evaluation of metronidazole-susceptible .
是全球最常见的性传播寄生虫。然而，日本不存在监测病例和耐药性的监测系统。
从有或无疑似感染症状的女性中收集宫颈细胞学阴道拭子；这些拭子用于通过特异性聚合酶链反应检测、人乳头瘤病毒（HPV）和。对临床分离株使用先前报道的最小致死浓度（MLC）和新建立的半数最大抑制浓度（IC）值进行甲硝唑敏感性试验。
研究人群中的感染率为4.2%（5/119；95%置信区间[Cl]，1.5 - 9.7）。此外，无症状感染占所有感染病例的60%（3/5）。所有阳性患者均合并感染HPV，但未合并感染。5株临床分离株对甲硝唑敏感，这是通过MLC值评估的。定量IC值显示，其中2株临床分离株对甲硝唑的敏感性降低。
这是第一项证明日本女性中感染率的研究。甲硝唑对的IC值能够对甲硝唑敏感的进行精确和定量评估。
