Dervišević Emina, Hasić Sabaheta, Katica Muhamed, Dervišević Lejla, Ajanović Zurifa, Salihbegović Adis
Department of Forensic Medicine, Faculty of Medicine, University of Sarajevo, 71 000 Sarajevo, Bosnia and Herzegovina.
Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Heliyon. 2023 Mar 25;9(4):e14565. doi: 10.1016/j.heliyon.2023.e14565. eCollection 2023 Apr.
Heat-related biomarkers: Focus on the correlation of troponin I and 70 kDa heat shock protein Abstract Introduction: There is intensive research related to the forensic importance of biomarkers that would be the standard for postmortem damage to cardiomyocytes and the mechanism of the resulting damage. The aim of the research was to examinate the forensic-medical significance of serum levels of biomarkers as detectors of terminal hyperthermic damage to the myocardium.
40 laboratory animals were divided into groups: the first group was the control (n = 8) exposed to a physiological temperature of 37 °C, the second group was divided into two subgroups: antemortem (n = 8) and postmortem (n = 8), which included a exposure temperature of 41 °C and the third group was divided into two subgroups: antemortem (n = 8) and postmortem (n = 8), which included a exposure temperature of 44 °C. The concentration of cardiac TnI and Hsp70 was resoluted in serum by immunochemical enzyme-labeled immunoabsorption method.
A positive correlation was found between the temperature measured at the time of death and the serum values of cTnI (p = 0.02), in G41, and Hsp70 values did not significantly correlate with the core temperature in this group, p > 0.005. A positive correlation was significant between the concentration of Hsp 70 and the body temperature of rats in the group of rats with a fatal outcome was determined, p = 0.03.
Changes in the concentration of cTnI and Hsp70 in rat serum may indicate hyperthermic damage to the myocardium in the Wistar rat model of heat stroke.
热相关生物标志物：关注肌钙蛋白I与70 kDa热休克蛋白的相关性 摘要 引言：关于生物标志物在法医学上的重要性有大量研究，这些生物标志物将成为心肌细胞死后损伤的标准以及由此产生损伤的机制。该研究的目的是检验作为心肌终末热损伤检测指标的生物标志物血清水平的法医学意义。
40只实验动物被分为几组：第一组为对照组（n = 8），暴露于37°C的生理温度下；第二组分为两个亚组：生前组（n = 8）和死后组（n = 8），暴露温度为41°C；第三组分为两个亚组：生前组（n = 8）和死后组（n = 8），暴露温度为44°C。通过免疫化学酶标免疫吸附法测定血清中心脏肌钙蛋白I（TnI）和热休克蛋白70（Hsp70）的浓度。
在G41组中，死亡时测得的温度与cTnI的血清值之间存在正相关（p = 0.02），且该组中Hsp70值与核心温度无显著相关性，p > 0.005。在确定有致命结果的大鼠组中，Hsp 70浓度与大鼠体温之间存在显著正相关，p = 0.03。
大鼠血清中cTnI和Hsp70浓度的变化可能表明在Wistar大鼠热中风模型中心肌发生了热损伤。
