Odumosu Bamidele Tolulope, Bamidele Tajudeen Akanji, Ofem Daniella Williams, Agbozo Friday, Olasehinde Grace I
Department of Microbiology, University of Lagos, Akoka-yaba, Nigeria.
Department of Molecular Biology and Biotechnology, Nigeria Institute of Medical Research Lagos, Nigeria.
Heliyon. 2023 Mar 28;9(4):e14959. doi: 10.1016/j.heliyon.2023.e14959. eCollection 2023 Apr.
Vitamins are an essential food source with excellent roles in the cellular metabolism and other essential nutrients required in food intake but cannot be synthesized by humans. There have been reports of some lactic acid bacteria (LAB) abilities with probiotic activities to produce food-grade vitamins. Our study aimed to investigate lactic acid bacteria (LAB) possessing antimicrobial activities and extracellular production of folate from different Nigerian fermented foods. LAB was assayed for their antimicrobial activities against clinical isolates of and and their extracellular production of essential vitamins. Among the 43 isolates of LAB, two strains of showed the highest inhibitions against the test bacteria and demonstrated the highest concentrations of extracellular vitamins production. The range of vitamins produced at 24 h was between 12.23 and 801.79 μg/ml, while the highest vitamin production of 801.79 and 310.55 μg/ml was observed for folate and vitamin B12 respectively, the lowest production was for B1+B2. Consistent vitamin production was typical with only MT903311 and MT903312, so were their antimicrobial activities. The L. strains isolated in this study could be exploited and applied in food products to substitute synthetic vitamin enrichment and fortification.
维生素是一种必需的食物来源，在细胞代谢中起着重要作用，是食物摄入所需的其他必需营养素，但人体无法合成。有报道称，一些具有益生菌活性的乳酸菌有能力生产食品级维生素。我们的研究旨在调查来自尼日利亚不同发酵食品的具有抗菌活性和胞外产生叶酸能力的乳酸菌。对乳酸菌进行了针对金黄色葡萄球菌和大肠杆菌临床分离株的抗菌活性及其必需维生素胞外产生情况的测定。在43株乳酸菌分离物中，两株嗜酸乳杆菌对测试细菌表现出最高的抑制作用，并显示出最高浓度的胞外维生素产生。24小时产生的维生素范围在12.23至801.79μg/ml之间，其中叶酸和维生素B12的最高产量分别为801.79和310.55μg/ml，B1+B2的产量最低。只有嗜酸乳杆菌MT903311和嗜酸乳杆菌MT903312具有典型的持续维生素产生能力，它们的抗菌活性也是如此。本研究中分离出的嗜酸乳杆菌菌株可用于食品产品，以替代合成维生素的强化和添加。
